HSP21 Antibody

Code CSB-PA327028XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HSP21 Polyclonal antibody
Uniprot No.
Target Names
HSP21
Alternative Names
HSP21 antibody; HSP25.3 antibody; At4g27670 antibody; T29A15.160 antibody; Heat shock protein 21 antibody; chloroplastic antibody; 25.3 kDa heat shock protein antibody; chloroplastic antibody; AtHsp25.3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana HSP21 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Chaperone protein required for seedling and chloroplast development under heat stress, probably by maintaining plastid-encoded RNA polymerase (PEP)-dependent transcription.
Gene References into Functions
  1. plastid-localized metalloprotease FtsH6 regulates HSP21 abundance. Lack of a functional FtsH6 protein promotes HSP21 accumulation during the later stages of thermomemory and increases thermomemory capacity. PMID: 27561243
  2. Findings reveal an auto-adaptation loop pathway that plant cells optimize particular needs of chloroplasts in stabilizing photosynthetic complexes by relaying the GUN5-dependent plastid signal(s) to activate the heat-responsive expression of HSP21 in the nucleus during adaptation to heat stress in plants. PMID: 27943531
  3. Studied Hsp21 association with thylakoid membranes in heat stress resistance of Arabidopsis thaliana. PMID: 28608391
  4. analysis of the structure of dodecameric heat-shock protein Hsp21 PMID: 28325834
  5. Present a structure model of Arabidopsis thaliana Hsp21, obtained by homology modeling, single-particle electron microscopy, and lysine-specific chemical crosslinking. PMID: 21280121
  6. evolution of a pseudogene: diversity and divergence of HSP21 PMID: 18356953

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Subcellular Location
Plastid, chloroplast stroma, chloroplast nucleoid.
Protein Families
Small heat shock protein (HSP20) family
Tissue Specificity
Confined to pollen grains of budding flowers.
Database Links

KEGG: ath:AT4G27670

STRING: 3702.AT4G27670.1

UniGene: At.5368

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