Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Kpnb1 Polyclonal antibody

Uniprot No.

P70168

Target Names

Kpnb1

Alternative Names

Kpnb1 antibody; Impnb antibody; Importin subunit beta-1 antibody; Karyopherin subunit beta-1 antibody; Nuclear factor p97 antibody; Pore targeting complex 97 kDa subunit antibody; PTAC97 antibody; SCG antibody

Raised in

Rabbit

Species Reactivity

Mus musculus

Immunogen

Recombinant Mus musculus Kpnb1 protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (12-14 weeks)

Target Background

Function

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Imports SNAI1 and PRKCI into the nucleus.

Gene References into Functions

KPNb1 acts as a positive regulator in the NF-kappaB pathway to enhance palmitate-induced inflammation response. PMID: 26452501
KPNB1 may play a key role in the inflammation process of rheumatoid arthritis via STAT3 signal transduction pathway. PMID: 26879143
The study revealed a regulatory role of the p97-Npl4-Ufd1 complex in regulating a partial degradation of the NF-kappaB subunit p100. PMID: 26112410
Ei24 can bind specifically to IMPbeta1 and IMPalpha2 to impede their normal role in nuclear import. PMID: 24821838
Identification of a karyopherin beta1/beta2 proline-tyrosine nuclear localization signal in huntingtin protein. PMID: 23012356
translation of Importin beta1 mRNA enables separation of cytoplasmic and nuclear transport functions of importins and is required for efficient retrograde signaling in injured axons. PMID: 22841314
A critical function of RanBP2 is to capture recycling RanGTP-importin-beta complexes at cytoplasmic fibrils to allow for adequate classical nuclear localization signal-mediated cargo import. PMID: 21859863
Ap4A hydrolase was found to translocate into the nuclei of mast cells following immunological activation; found its immunologically dependent association with importin beta PMID: 21402779
Data show that MRTF-A contains an unusually long bipartite nuclear localisation signal embedded within the RPEL domain, that uses the importin (Imp)alpha/beta-dependent import pathway, and that import is inhibited by G-actin. PMID: 20818336
results show the crystal structure of importin-beta complexed with the active form of SREBP-2 PMID: 14645851
Results suggest that the importin alpha/beta system is involved in nuclear entry of mammalian clock components Cry2 and Per2, which is indispensable to transcriptional oscillation of clock genes PMID: 15689618
Importin-beta features many more binding spots for nucleoporins than suspected so far. PMID: 16338415
These results demonstrated that the cause of post-implantation lethality of Ayu8108(geo/geo) homozygous embryos was impaired expression of the Kpnb1 gene. PMID: 16414015
PTH1R forms a complex with the transport regulatory proteins, importin alpha(1) and importin beta, and nuclear PTH1R is associated with the nucleoplasm PMID: 16574786
Importin beta1 protein is detected in selected germ and somatic cells in fetal gonads, with a striking perinuclear staining evident from embryonic day (E) 14.5 within testicular gonocytes PMID: 17654710
reduction of the affinity by RanGTP really occurs at the nucleoplasmic side of the entire nuclear pore complex PMID: 18845677
Dtaa show that importin beta and the integral nuclear pore glycoprotein Nup62 interact with hsp90, hsp70, p23, and the TPR domain proteins FKBP52 and PP5 during nuclear transport. PMID: 19581287

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Subcellular Location

Cytoplasm. Nucleus envelope.

Protein Families

Importin beta family, Importin beta-1 subfamily

Database Links

KEGG: mmu:16211

STRING: 10090.ENSMUSP00000001479

UniGene: Mm.251013

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Kpnb1 Antibody

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