MBD4 Antibody

Code CSB-PA888863XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MBD4 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
MBD4 antibody; At3g63030 antibody; T20O10.130Methyl-CpG-binding domain-containing protein 4 antibody; AtMBD4 antibody; MBD04 antibody; Methyl-CpG-binding protein MBD4 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MBD4 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Transcriptional regulator that binds CpG, CpNpN and CpNpG (N is A, T, or C) islands in promoters regardless the DNA methylation status. Plays probably a role in gene silencing.
Gene References into Functions
  1. These results suggest that endogenous MBD4L acts on particular tissues, is capable of activating base excision repair, and may contribute to repair DNA damage caused by oxidative stress. PMID: 25900572
  2. Results suggest that an important function of Arabidopsis MBD4L is to protect the plant genome from the mutagenic consequences of cytosine and 5-methylcytosine deamination. PMID: 23994068
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in rosette leaves, buds, flowers, stems, mature seeds and roots.
Database Links

KEGG: ath:AT3G63030

STRING: 3702.AT3G63030.1

UniGene: At.66481

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