Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MCM2 Polyclonal antibody

Uniprot No.

P29469

Target Names

MCM2

Alternative Names

MCM2 antibody; YBL023C antibody; YBL0438DNA replication licensing factor MCM2 antibody; EC 3.6.4.12 antibody; Minichromosome maintenance protein 2 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MCM2 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity; specifically the MCM2-MCM5 association is proposed to be reversible and to mediate a open ring conformation which may facilitate DNA loading. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity. Necessary for cell growth.

Gene References into Functions

An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-alpha to Mcm2-7. PMID: 28510759
Mcm10 directly binds the Minichromosome Maintenance Complex via Mcm2 and the Mcm6 C terminus. PMID: 26719337
Mcm2-7 and specifically the ATPase site inactivated by mcm2DENQ are required at an intermediate step of the DNA replication checkpoint signal transduction cascade. PMID: 25870112
Mcm2-7 phosphorylation and firing factor recruitment promote complete and semi-conservative replication of the input plasmids. PMID: 24566989
The data demonstrate that ORC/Cdc6 facilitates directly MCM2-7 dimerization and shows that productive MCM2-7 dimerization is essential for prereplicative complex formation. PMID: 24234446
Mcm2 binds histones together with FACT and contributes to the efficacy of chromatin replication. PMID: 23499444
Data indicate that Cdt1-MCM2-7 complex activates ORC/Cdc6 ATP-hydrolysis to promote helicase loading. PMID: 23603117
constitutive overexpression of each individual MCM subunit was examined, and genotoxic sensitivity was found to be specific to Mcm2 or Mcm4 overexpression, further pointing to the importance of the DDK-MCM ring interaction. PMID: 23549044
A role for DDK-mediated phosphorylation of Mcm2 modulates Mcm2-7 activity resulting in the stabilization of replication. PMID: 22564307
origin ssDNA may stimulate the formation of the CMG complex by alleviating inhibitory interactions between Sld2 with Mcm2-7. PMID: 21868389
origin single-stranded DNA releases Sld3 from Mcm2-7, allowing GINS to bind Mcm2-7. PMID: 21460226
these results indicate that the nucleotide-bound state of Mcm2 is critical in regulating the activities of Mcm4/6/7 and Mcm2-7 complexes. PMID: 19540846
Data indicate that a complex of the GINS and Ctf4 components of the replisome progression complex is crucial to couple MCM2-7 to DNA polymerase alpha. PMID: 19661920
Study shows that DNA runs through a central channel in the double hexamer, and, once loaded, Mcm2-7 can slide passively along double-stranded DNA. PMID: 19896182

Hide All

Subcellular Location

Nucleus.

Protein Families

MCM family

Database Links

KEGG: sce:YBL023C

STRING: 4932.YBL023C

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MCM2 Antibody

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