mreB Antibody

Datasheet

Code	CSB-PA359362XA01ENV
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: mreB Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa
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Target Background

Product Details

Uniprot No.

P0A9X4

Target Names

mreB

Alternative Names

Rod shape-determining protein MreB mreB envB rodY b3251 JW3220

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) mreB protein (1-347aa)

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The generation of polyclonal antibodies against mreB encompasses the iterative immunization of a rabbit with recombinant Escherichia coli (strain K12) mreB protein (1-347aa). After attaining a sufficient antibody titer, the rabbit is bled, and antibodies are purified from the serum using affinity chromatography. Subsequent validation of the mreB antibody's functionality occurs through ELISA and WB applications, showcasing its reactivity with Escherichia coli (strain K12) mreB protein.

The mreB protein in Escherichia coli (strain K12) plays a central role in the bacterial cytoskeleton, functioning akin to eukaryotic actin. Its primary role lies in the maintenance of cell shape and the establishment of cell morphology by organizing into helical filaments beneath the cell membrane. As a key orchestrator of the spatial organization of enzymes involved in peptidoglycan synthesis, mreB guides the incorporation of new cell wall material along the cell length, ensuring the rod-like shape of bacterial cells.

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Target Background

Function

Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization maintains elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature. Filaments rotate around the cell circumference in concert with the cell wall synthesis enzymes. The process is driven by the cell wall synthesis machinery and does not depend on MreB polymerization. Rotation may contribute to the robust maintenance of rod shape.

Subcellular Location

Cytoplasm.

Protein Families

FtsA/MreB family

Database Links

KEGG: ecj:JW3220

STRING: 316385.ECDH10B_3427

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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mreB Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background