N Antibody

Datasheet

Code	CSB-PA320135ZA01ARV
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: N antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45.9 kDa Observed band size: 55 kDa
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Target Background

Product Details

Uniprot No.

P12648

Target Names

Alternative Names

Nucleoprotein (Nucleocapsid protein) (NC) (Protein N), N

Species Reactivity

Avian infectious bronchitis virus

Immunogen

Recombinant Avian infectious bronchitis virus N protein (1-409aa)

Immunogen Species

Avian infectious bronchitis virus

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The generation of the nucleoprotein (N) polyclonal antibody initiates with the use of a recombinant Avian infectious bronchitis virus N protein segment (encompassing amino acids 1-409) as the immunogen, administered to a rabbit to induce an antibody response. Serum drawn from the rabbit contains polyclonal antibodies, which are subsequently purified via protein G. This N antibody's functional utility is established through ELISA and WB assays, affirming its proficiency in detecting Avian infectious bronchitis virus N protein in experimental settings.

Avian infectious bronchitis virus (IBV) is a gamma coronavirus that causes acute and highly contagious upper respiratory tract disease in avian species worldwide. The IBV N protein is a non-specific RNA-binding protein that is vital for the replication, transcription, assembly, and protection of the viral RNA, contributing to the overall success of the virus's life cycle and pathogenicity.

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Target Background

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

Subcellular Location

Virion. Host endoplasmic reticulum-Golgi intermediate compartment. Host Golgi apparatus.

Protein Families

Gammacoronavirus nucleocapsid protein family

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