Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Neil3 Polyclonal antibody

Uniprot No.

Q8K203

Target Names

Neil3

Alternative Names

Neil3 antibody; Endonuclease 8-like 3 antibody; EC 3.2.2.- antibody; EC 4.2.99.18 antibody; DNA glycosylase FPG2 antibody; DNA glycosylase/AP lyase Neil3 antibody; Endonuclease VIII-like 3 antibody; Nei-like protein 3 antibody

Raised in

Rabbit

Species Reactivity

Mus musculus (Mouse)

Immunogen

Recombinant Mus musculus (Mouse) Neil3 protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). Mediates interstrand cross-link repair in response to replication stress: acts by mediating DNA glycosylase activity, cleaving one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases. In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues.

Gene References into Functions

Results suggest a novel role for the DNA glycosylase Neil3 in atherogenesis in balancing lipid metabolism and macrophage function, potentially independently of genome-wide canonical base excision repair of oxidative DNA damage. PMID: 27328939
Data suggest that neurogenesis induced by Neil3 repair of oxidative DNA damage protects against prion disease during the clinical phase. PMID: 27886261
NEIL3-dependent modulation of DNA methylation regulates cardiac fibroblast proliferation and thereby affects extracellular matrix modulation after myocardial infarction. PMID: 28052262
These findings demonstrate that deficiency in NEIL3 is associated with increased lymphocyte apoptosis, autoantibodies, and predisposition to autoimmunity. PMID: 27760045
Structural insight into the substrate specificity and marked preference of Neil3 for ssDNA. PMID: 23313161
Our data support the involvement of Neil3 in removal of replication blocks in proliferating cells. PMID: 23305905
Neil3-dependent repair of oxidative DNA damage in neural stem/progenitor cells is required for maintenance of adult neurogenesis to counteract the age-associated deterioration of cognitive performance. PMID: 22959434
Here we report the construction of bicistronic expression vectors for expressing in Escherichia coli the full-length mouse Neil3 (MmuNeil3), its glycosylase domain (MmuNeil3Delta324), as well as the glycosylase domain of human Neil3 (NEIL3Delta324). PMID: 22569481
Neil3 exercises a highly specialized function through accurate molecular repair of DNA in rapidly proliferating cells PMID: 22065741
Neil3 plays a role in repairing 2,6-diamino-4-hydroxy-5-formamidopyrimidine in vivo. PMID: 20185759
NEIL3 is localized in the nuclei; Neil3 mRNA was selectively expressed in thymus, spleen and bone marrow PMID: 16428305
NEIL3 is specifically expressed in brain areas where neurogenesis takes place during development and that its expression is tightly regulated both temporally and spatially. NEIL3 seems to be upregulated in tumor tissues compared to normal tissues. PMID: 19426544

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Subcellular Location

Nucleus. Chromosome.

Protein Families

FPG family

Tissue Specificity

Expressed in testis, thymus, spleen and bone marrow. In young mice, expressed at higher levels in thymocytes than splenocytes. At 12 dpc, abundant in the subventricular zone (SVZ) of the lateral ventricles. At 17.5 dpc and P0, expression is limited to dis

Database Links

KEGG: mmu:234258

STRING: 10090.ENSMUSP00000041909

UniGene: Mm.281749

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Neil3 Antibody

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