Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) OPR3 Polyclonal antibody

Uniprot No.

Q9FUP0

Target Names

OPR3

Alternative Names

OPR3 antibody; DDE1 antibody; At2g06050 antibody; F5K7.19 antibody; 12-oxophytodienoate reductase 3 antibody; EC 1.3.1.42 antibody; 12-oxophytodienoate-10,11-reductase 3 antibody; AtOPR3 antibody; OPDA-reductase 3 antibody; Protein DELAYED DEHISCENCE 1) [Cleaved into: 12-oxophytodienoate reductase 3 antibody; N-terminally processed] antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana OPR3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Specifically cleaves olefinic bonds in cyclic enones. Involved in the biosynthesis of jasmonic acid (JA) and perhaps in biosynthesis or metabolism of other oxylipin signaling moleclules. Required for the spatial and temporal regulation of JA levels during dehiscence of anthers, promoting the stomium degeneration program. In vitro, reduces 9S,13S-12-oxophytodienoic acid (9S,13S-OPDA) and 9R,13R-OPDA to 9S,13S-OPC-8:0 and 9R,13R-OPC-8:0, respectively. Can detoxify the explosive 2,4,6-trinitrotoluene (TNT) in vitro by catalyzing its nitroreduction to form hydroxylamino-dinitrotoluene (HADNT).

Gene References into Functions

The authors found that OPR3 specifically inhibited primary root growth under phosphate deficiency via suppressing root tip growth at the transcriptional level, revealing an important novel function of OPR3 in regulating primary root response to the nutrient stress. PMID: 27101793
In structures of substrate-free and 8-iso-PGA1 bound AtOPR3, the two monomers observed in the asymmetric unit of AtOPR3 crystals can be superimposed with the corresponding protomers of the LeOPR3 dimer, suggesting a similar dimeric structure for AtOPR3. PMID: 21915915
Over-expression of OPR3 does not strongly induce BPEp accumulation, but exogenous application of jasmonate restores BPEp expression in the opr3 mutant. PMID: 19765234
OPR3 was found to be induced by touch, wounding, methyl jasmonate, NaCl and CaCl(2) while ethylene and darkness had no effect. PMID: 16533544
brassinosteroid-responsive gene PMID: 16807820

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Subcellular Location

Peroxisome.

Protein Families

NADH:flavin oxidoreductase/NADH oxidase family

Tissue Specificity

Expressed in green seedling, leaves, flowers (anthers, pistil, petal and stamen), and to a lower extent in roots and siliques. Specifically expressed in filament during anther dehiscence initiation.

Database Links

KEGG: ath:AT2G06050

STRING: 3702.AT2G06050.1

UniGene: At.1135

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OPR3 Antibody

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