Recombinant Arabidopsis thaliana 12-oxophytodienoate reductase 3 (OPR3)

Code CSB-YP884329DOA
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Source Yeast
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Code CSB-EP884329DOA
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Source E.coli
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Code CSB-EP884329DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP884329DOA
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Source Baculovirus
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Code CSB-MP884329DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
OPR3
Uniprot No.
Alternative Names
OPR3; DDE1; At2g06050; F5K7.19; 12-oxophytodienoate reductase 3; EC 1.3.1.42; 12-oxophytodienoate-10,11-reductase 3; AtOPR3; OPDA-reductase 3; Protein DELAYED DEHISCENCE 1) [Cleaved into: 12-oxophytodienoate reductase 3; N-terminally processed]
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-391
Target Protein Sequence
MTAAQGNSNE TLFSSYKMGR FDLSHRVVLA PMTRCRALNG VPNAALAEYY AQRTTPGGFL ISEGTMVSPG SAGFPHVPGI YSDEQVEAWK QVVEAVHAKG GFIFCQLWHV GRASHAVYQP NGGSPISSTN KPISENRWRV LLPDGSHVKY PKPRALEASE IPRVVEDYCL SALNAIRAGF DGIEIHGAHG YLIDQFLKDG INDRTDQYGG SIANRCRFLK QVVEGVVSAI GASKVGVRVS PAIDHLDATD SDPLSLGLAV VGMLNKLQGV NGSKLAYLHV TQPRYHAYGQ TESGRQGSDE EEAKLMKSLR MAYNGTFMSS GGFNKELGMQ AVQQGDADLV SYGRLFIANP DLVSRFKIDG ELNKYNRKTF YTQDPVVGYT DYPFLAPFSR L
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Specifically cleaves olefinic bonds in cyclic enones. Involved in the biosynthesis of jasmonic acid (JA) and perhaps in biosynthesis or metabolism of other oxylipin signaling moleclules. Required for the spatial and temporal regulation of JA levels during dehiscence of anthers, promoting the stomium degeneration program. In vitro, reduces 9S,13S-12-oxophytodienoic acid (9S,13S-OPDA) and 9R,13R-OPDA to 9S,13S-OPC-8:0 and 9R,13R-OPC-8:0, respectively. Can detoxify the explosive 2,4,6-trinitrotoluene (TNT) in vitro by catalyzing its nitroreduction to form hydroxylamino-dinitrotoluene (HADNT).
Gene References into Functions
  1. The authors found that OPR3 specifically inhibited primary root growth under phosphate deficiency via suppressing root tip growth at the transcriptional level, revealing an important novel function of OPR3 in regulating primary root response to the nutrient stress. PMID: 27101793
  2. In structures of substrate-free and 8-iso-PGA1 bound AtOPR3, the two monomers observed in the asymmetric unit of AtOPR3 crystals can be superimposed with the corresponding protomers of the LeOPR3 dimer, suggesting a similar dimeric structure for AtOPR3. PMID: 21915915
  3. Over-expression of OPR3 does not strongly induce BPEp accumulation, but exogenous application of jasmonate restores BPEp expression in the opr3 mutant. PMID: 19765234
  4. OPR3 was found to be induced by touch, wounding, methyl jasmonate, NaCl and CaCl(2) while ethylene and darkness had no effect. PMID: 16533544
  5. brassinosteroid-responsive gene PMID: 16807820

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Subcellular Location
Peroxisome.
Protein Families
NADH:flavin oxidoreductase/NADH oxidase family
Tissue Specificity
Expressed in green seedling, leaves, flowers (anthers, pistil, petal and stamen), and to a lower extent in roots and siliques. Specifically expressed in filament during anther dehiscence initiation.
Database Links

KEGG: ath:AT2G06050

STRING: 3702.AT2G06050.1

UniGene: At.1135

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