flw Antibody

Code CSB-PA344529XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) flw Polyclonal antibody
Uniprot No.
Target Names
flw
Alternative Names
flw antibody; PP1-9C antibody; PP1-BETA-9C antibody; CG2096 antibody; Serine/threonine-protein phosphatase beta isoform antibody; EC 3.1.3.16 antibody; Protein flap wing antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) flw protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for cell adhesion in non-muscle tissues and in maintenance of muscle attachment. Vital for larval development.
Gene References into Functions
  1. Flw functions together with myosin phosphatase 1beta to negatively regulate myosin activity during ring canal formation in oogenesis. PMID: 23936219
  2. identified a loss-of-function mutation of flapwing, which encodes the catalytic subunit of protein phosphatase 1beta (PP1beta) that disrupts oocyte polarization PMID: 21490061
  3. nuclear inhibitor of protein phosphatase type 1 (NIPP1) and protein phosphatase type 1 (PP1c) interact in vivo in Drosophila PMID: 12358598
  4. Data show that Drosophila PP1beta, a specific isoform of serine/threonine protein phosphatase 1 (PP1), regulates nonmuscle myosin and that this is the essential role of PP1beta. PMID: 15269282
  5. a Jun N-terminal kinase (JNK) mutant suppresses the semilethality of a strong flw allele PMID: 17277363

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Protein Families
PPP phosphatase family, PP-1 subfamily
Database Links

KEGG: dme:Dmel_CG2096

STRING: 7227.FBpp0071381

UniGene: Dm.7222

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