Recombinant Drosophila melanogaster Serine/threonine-protein phosphatase beta isoform (flw)

Code CSB-YP344529DLU
MSDS
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Source Yeast
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Code CSB-EP344529DLU
MSDS
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Source E.coli
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Code CSB-EP344529DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP344529DLU
MSDS
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Source Baculovirus
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Code CSB-MP344529DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
flw
Uniprot No.
Alternative Names
flw; PP1-9C; PP1-BETA-9C; CG2096; Serine/threonine-protein phosphatase beta isoform; EC 3.1.3.16; Protein flap wing
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-330
Target Protein Sequence
MGDFDLNVDS LIQRLLEMRS CRTGKQVQMT EAEVRGLCLK SREIFLQQPI LLELEAPLII CGDIHGQYTD LLRLFEYGGF PPAANYLFLG DYVDRGKQSL ETICLLLAYK IKYPENFFLL RGNHECASIN RIYGFYDECK RRYNVKLWKT FTDCFNCLPV AAIIDEKIFC CHGGLSPDLQ GMEQIRRLMR PTDVPDTGLL CDLLWSDPDK DVQGWGENDR GVSFTFGVDV VSKFLNRHEL DLICRAHQVV EDGYEFFARR QLVTLFSAPN YCGEFDNAGG MMTVDDTLMC SFQILKPSEK KAKYLYSGMN SSRPTTPQRS APMLATNKKK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for cell adhesion in non-muscle tissues and in maintenance of muscle attachment. Vital for larval development.
Gene References into Functions
  1. Flw functions together with myosin phosphatase 1beta to negatively regulate myosin activity during ring canal formation in oogenesis. PMID: 23936219
  2. identified a loss-of-function mutation of flapwing, which encodes the catalytic subunit of protein phosphatase 1beta (PP1beta) that disrupts oocyte polarization PMID: 21490061
  3. nuclear inhibitor of protein phosphatase type 1 (NIPP1) and protein phosphatase type 1 (PP1c) interact in vivo in Drosophila PMID: 12358598
  4. Data show that Drosophila PP1beta, a specific isoform of serine/threonine protein phosphatase 1 (PP1), regulates nonmuscle myosin and that this is the essential role of PP1beta. PMID: 15269282
  5. a Jun N-terminal kinase (JNK) mutant suppresses the semilethality of a strong flw allele PMID: 17277363

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Protein Families
PPP phosphatase family, PP-1 subfamily
Database Links

KEGG: dme:Dmel_CG2096

STRING: 7227.FBpp0071381

UniGene: Dm.7222

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