crr Antibody

^{Rare Species}

Datasheet

Code	CSB-PA302513XA01ENV
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: crr Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 33 kDa Observed band size: 33 kDa
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Product Details

Uniprot No.

P69783

Target Names

crr

Alternative Names

PTS system glucose-specific EIIA component (EIIA-Glc) (EIII-Glc) (Glucose-specific phosphotransferase enzyme IIA component) (EC 2.7.1.199) crr gsr iex tgs treD b2417 JW2410

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) crr protein (12-67aa)

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The generation of polyclonal antibodies against crr entails repeated immunizations of a rabbit with recombinant Escherichia coli (strain K12) crr protein (12-67aa). After achieving a sufficient antibody titer, the rabbit is bled, and antibodies are purified from the serum through affinity chromatography. The resulting crr antibody is then tested for functionality in ELISA and WB applications, showing reactivity with Escherichia coli (strain K12) crr protein.

The Escherichia coli (strain K12) crr protein is a crucial component of the phosphoenolpyruvate (PEP): carbohydrate phosphotransferase system (PTS), a major regulatory mechanism governing carbohydrate transport and utilization. Crr functions as a phospho-carrier protein, facilitating the transfer of phosphoryl groups from PEP to incoming sugar substrates, a pivotal step in sugar uptake by the bacterial cell. Beyond its role in sugar phosphotransferase activity, crr is instrumental in the regulation of carbohydrate uptake, responding dynamically to changes in the availability of sugars in the environment.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II complex composed of PtsG and Crr is involved in glucose transport. The non-phosphorylated EIII-Glc is an inhibitor for uptake of certain sugars such as maltose, melibiose, lactose, and glycerol. Phosphorylated EIII-Glc, however, may be an activator for adenylate cyclase. It is an important regulatory protein for cell metabolism.

Gene References into Functions

Results suggest that the phosphoenolpyruvate hexose phosphotransferases PTS network serves as a global sensor of the sugar influx and also monitors the levels of several non-PTS compounds. PMID: 27557415
results of a detailed investigation of the role of EIIAGlc in CsrB/C decay; unphosphorylated EIIAGlc binds specifically to the EAL domain of CsrD and stimulates CsrB/C turnover PMID: 26507976
The data suggest a model of how the central regulatory protein EIIA(Glc) allosterically inhibits maltose uptake in E. coli. PMID: 23770568

Subcellular Location

Cytoplasm.

Database Links

KEGG: ecj:JW2410

STRING: 316385.ECDH10B_2582

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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crr Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background