Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Pla2g15 Polyclonal antibody

Uniprot No.

Q8VEB4

Target Names

Pla2g15

Alternative Names

Pla2g15 antibody; Lypla3 antibody; Group XV phospholipase A2 antibody; EC 2.3.1.- antibody; 1-O-acylceramide synthase antibody; ACS antibody; LCAT-like lysophospholipase antibody; LLPL antibody; Lysophospholipase 3 antibody; Lysosomal phospholipase A2 antibody; LPLA2 antibody

Raised in

Rabbit

Species Reactivity

Mus musculus

Immunogen

Recombinant Mus musculus Pla2g15 protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (12-14 weeks)

Target Background

Function

Has dual calcium-independent phospholipase and O-acyltransferase activities with a potential role in glycerophospholipid homeostasis and remodeling of acyl groups of lipophilic alcohols present in acidic cellular compartments. Catalyzes hydrolysis of the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 or A2 activity) and transfer it to the hydroxyl group at the first carbon of lipophilic alcohols (O-acyltransferase activity). Among preferred fatty acyl donors are phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols and phosphatidylserines. Favors sn-2 over sn-1 deacylation of unsaturated fatty acyl groups of phosphatidylcholines and phosphatidylethanolamines. Among preferred fatty acyl acceptors are natural lipophilic alcohols including short-chain ceramide N-acetyl-sphingosine (C2 ceramide), alkylacylglycerols, monoacylglycerols, and acylethanolamides such as anandamide and oleoylethanolamide. Selectively hydrolyzes the sn-1 fatty acyl group of truncated oxidized phospholipids and may play a role in detoxification of reactive oxidized phospholipids during oxidative stress. Required for normal phospholipid degradation in alveolar macrophages with potential implications in pulmonary surfactant clearance. At neutral pH, hydrolyzes the sn-1 fatty acyl group of the lysophosphatidylcholines.

Gene References into Functions

LPLA2 plays a role in the generation of CD1d complexes with thymic lipids required for the normal selection and maturation of iNKT cells. PMID: 23493550
LPLA2 may be a major enzyme of pulmonary surfactant phospholipid degradation by alveolar macrophages PMID: 15294901
activity is anti-atherogenic and indicate that the regulation of this enzyme might be a novel drug target for the treatment of atherosclerosis PMID: 15781238
identification of the catalytic triad and the role of cysteine residues in lysosomal phospholipase A2 PMID: 16106046
Lpla2 has broad positional specificity for the sn-1 and sn-2 acyl groups in phosphatidylcholine and phosphatidylethanolamine PMID: 16837646
A deficiency of lysosomal phospholipase A2 results in foam cell formation, surfactant lipid accumulation, splenomegaly, and phospholipidosis in mice. PMID: 16880524
The secretion and uptake of lysosomal phospholipase A2 by alveolar macrophages PMID: 19017977

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Subcellular Location

Secreted. Lysosome. Membrane; Peripheral membrane protein.

Protein Families

AB hydrolase superfamily, Lipase family

Tissue Specificity

Detected in blood plasma. Detected in alveolar macrophages (at protein level). Detected in heart, liver, spleen, kidney, thymus, brain and lung.

Database Links

KEGG: mmu:192654

STRING: 10090.ENSMUSP00000034377

UniGene: Mm.284770

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Pla2g15 Antibody

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