smad5 Antibody

Code CSB-PA894791XA01DIL
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) smad5 Polyclonal antibody
Uniprot No.
Target Names
smad5
Alternative Names
smad5 antibody; madh5 antibody; sbn antibody; Mothers against decapentaplegic homolog 5 antibody; MAD homolog 5 antibody; Mothers against DPP homolog 5 antibody; Protein somitabun antibody; SMAD family member 5 antibody; SMAD 5 antibody; Smad5 antibody
Raised in
Rabbit
Species Reactivity
Danio rerio (Zebrafish) (Brachydanio rerio)
Immunogen
Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) smad5 protein
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in ventralization. May mediate Bmp2b signaling during early phases of embryonic dorsal-ventral pattern formation. Required for initiation of Smad1 expression during gastrulation.
Gene References into Functions
  1. TGFbeta1a regulates zebrafish posterior lateral line formation via Smad5 mediated pathway. PMID: 25603803
  2. Alk3 and Alk3b, as well as SMAD5, are essential cellular mediators of BMP signaling in zebrfish. PMID: 24608800
  3. Data show that patterning of the eye primordia in Smad5-deficient embryos starts during blastula and early gastrula stages. PMID: 12701105
  4. Data show that Smad5 expression is ubiquitous during testis development but becomes cell-specific in the adult. PMID: 18069690
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Dwarfin/SMAD family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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