spoT Antibody

Datasheet

Code	CSB-PA360359XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) spoT Polyclonal antibody

Uniprot No.

P0AG24

Target Names

spoT

Alternative Names

spoT antibody; b3650 antibody; JW3625Bifunctional antibody; p)ppGpp synthase/hydrolase SpoT [Includes: GTP pyrophosphokinase antibody; EC 2.7.6.5; antibody; p)ppGpp synthase antibody; ATP:GTP 3'-pyrophosphotransferase antibody; Stringent response-like protein antibody; ppGpp synthase II); Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase antibody; EC 3.1.7.2 antibody; Penta-phosphate guanosine-3'-pyrophosphohydrolase; antibody; ppGpp)ase)] antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) spoT protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response which coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the synthesis and degradation of ppGpp. The second messengers ppGpp and c-di-GMP together control biofilm formation in response to translational stress; ppGpp represses biofilm formation while c-di-GMP induces it. ppGpp activates transcription of CsrA-antagonistic small RNAs CsrB and CsrC, which downregulate CsrA's action on translation during the stringent response.

Gene References into Functions

Dephosphorylated HPr, but not phosphorylated HPr, of the phosphoenolpyruvate-dependent sugar phosphotransferase system could antagonize the stimulatory effect of Rsd on SpoT (p)ppGpp hydrolase activity. PMID: 29915072
The results indicate that polyamine stimulation of synthesis of SpoT plays an important role for cell viability through stimulation of ppGpp synthesis by SpoT. PMID: 22138225
Data suggest that iron starvation produces a SpoT-dependent increase of the ppGpp pool, suggesting a role for iron in the balance of the two activities of SpoT, synthesis and hydrolysis of (p)ppGpp. PMID: 15853883
SpoT-mediated reduction of ppGpp triggers biofilm upregulation in response to translation inhibition. PMID: 19460094

Subcellular Location

Cytoplasm. Note=Is associated with pre-50S ribosomal subunits in a salt-dependent manner.

Protein Families

RelA/SpoT family

Database Links

KEGG: ecj:JW3625

STRING: 316385.ECDH10B_3832

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Email: [email protected]
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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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spoT Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background