Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

spoT

Uniprot No.

P0AG24

Research Area

Cancer

Alternative Names

(p)((p), (ATP:GTP 3'-pyrophosphotransferase)(Stringent response-like protein)(ppGpp synthase II)(diphosphate), (Penta-phosphate guanosine-3'-pyrophosphohydrolase)((ppGpp)

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

1-702aa

Target Protein Sequence

MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRVLGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENIQRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQEFITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

86.8 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Escherichia coli (strain K12) spoT covers amino acids 1-702. The calculated molecular weight for this spoT protein is 86.8 kDa. The spoT protein was expressed in e.coli. The spoT coding gene included the N-terminal 10xHis tag and C-terminal Myc tag, which simplifies the detection and purification processes of the recombinant spoT protein in following stages of expression and purification.

Escherichia coli SpoT is a bifunctional enzyme involved in the stringent response, a regulatory mechanism that enables bacteria to adapt to environmental stress conditions. SpoT possesses (p)ppGpp synthase and hydrolase activities, allowing it to control the levels of guanosine pentaphosphate and tetraphosphate [(p)ppGpp], collectively known as alarmones. During nutrient limitation or stress, SpoT synthesizes (p)ppGpp, signaling a global shift in cellular processes, including a slowdown in growth and changes in gene expression. Conversely, SpoT can hydrolyze (p)ppGpp when conditions become more favorable. This dual functionality allows SpoT to finely tune the bacterial response to varying environmental cues, contributing to bacterial survival and adaptation strategies.

Target Background

Function

In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response which coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the synthesis and degradation of ppGpp. The second messengers ppGpp and c-di-GMP together control biofilm formation in response to translational stress; ppGpp represses biofilm formation while c-di-GMP induces it. ppGpp activates transcription of CsrA-antagonistic small RNAs CsrB and CsrC, which downregulate CsrA's action on translation during the stringent response.

Gene References into Functions

Dephosphorylated HPr, but not phosphorylated HPr, of the phosphoenolpyruvate-dependent sugar phosphotransferase system could antagonize the stimulatory effect of Rsd on SpoT (p)ppGpp hydrolase activity. PMID: 29915072
The results indicate that polyamine stimulation of synthesis of SpoT plays an important role for cell viability through stimulation of ppGpp synthesis by SpoT. PMID: 22138225
Data suggest that iron starvation produces a SpoT-dependent increase of the ppGpp pool, suggesting a role for iron in the balance of the two activities of SpoT, synthesis and hydrolysis of (p)ppGpp. PMID: 15853883
SpoT-mediated reduction of ppGpp triggers biofilm upregulation in response to translation inhibition. PMID: 19460094

Subcellular Location

Cytoplasm. Note=Is associated with pre-50S ribosomal subunits in a salt-dependent manner.

Protein Families

RelA/SpoT family

Database Links

KEGG: ecj:JW3625

STRING: 316385.ECDH10B_3832

Code	CSB-EP360359ENV
Abbreviation	Recombinant E.coli spoT protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Escherichia coli Bifunctional (p)ppGpp synthase/hydrolase SpoT (spoT)

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