Recombinant Escherichia coli Bifunctional (p)ppGpp synthase/hydrolase SpoT (spoT)

In Stock
Code CSB-EP360359ENV
Size $388
Order now
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Greater than 85% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Alternative Names
(p)((p), (ATP:GTP 3'-pyrophosphotransferase)(Stringent response-like protein)(ppGpp synthase II)(diphosphate), (Penta-phosphate guanosine-3'-pyrophosphohydrolase)((ppGpp)
Escherichia coli (strain K12)
Expression Region
Target Protein Sequence
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
86.8 kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

The expression region of this recombinant Escherichia coli (strain K12) spoT covers amino acids 1-702. The calculated molecular weight for this spoT protein is 86.8 kDa. The spoT protein was expressed in e.coli. The spoT coding gene included the N-terminal 10xHis tag and C-terminal Myc tag, which simplifies the detection and purification processes of the recombinant spoT protein in following stages of expression and purification.

Escherichia coli SpoT is a bifunctional enzyme involved in the stringent response, a regulatory mechanism that enables bacteria to adapt to environmental stress conditions. SpoT possesses (p)ppGpp synthase and hydrolase activities, allowing it to control the levels of guanosine pentaphosphate and tetraphosphate [(p)ppGpp], collectively known as alarmones. During nutrient limitation or stress, SpoT synthesizes (p)ppGpp, signaling a global shift in cellular processes, including a slowdown in growth and changes in gene expression. Conversely, SpoT can hydrolyze (p)ppGpp when conditions become more favorable. This dual functionality allows SpoT to finely tune the bacterial response to varying environmental cues, contributing to bacterial survival and adaptation strategies.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response which coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the synthesis and degradation of ppGpp. The second messengers ppGpp and c-di-GMP together control biofilm formation in response to translational stress; ppGpp represses biofilm formation while c-di-GMP induces it. ppGpp activates transcription of CsrA-antagonistic small RNAs CsrB and CsrC, which downregulate CsrA's action on translation during the stringent response.
Gene References into Functions
  1. Dephosphorylated HPr, but not phosphorylated HPr, of the phosphoenolpyruvate-dependent sugar phosphotransferase system could antagonize the stimulatory effect of Rsd on SpoT (p)ppGpp hydrolase activity. PMID: 29915072
  2. The results indicate that polyamine stimulation of synthesis of SpoT plays an important role for cell viability through stimulation of ppGpp synthesis by SpoT. PMID: 22138225
  3. Data suggest that iron starvation produces a SpoT-dependent increase of the ppGpp pool, suggesting a role for iron in the balance of the two activities of SpoT, synthesis and hydrolysis of (p)ppGpp. PMID: 15853883
  4. SpoT-mediated reduction of ppGpp triggers biofilm upregulation in response to translation inhibition. PMID: 19460094
Subcellular Location
Cytoplasm. Note=Is associated with pre-50S ribosomal subunits in a salt-dependent manner.
Protein Families
RelA/SpoT family
Database Links
CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
Place an order now

I. Product details


II. Contact details


III. Ship To


IV. Bill To