GTF2H2 Antibody

Code CSB-PA107974XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GTF2H2 Polyclonal antibody
Uniprot No.
Target Names
GTF2H2
Alternative Names
GTF2H2 antibody; P44 antibody; At1g05055 antibody; T7A14.8 antibody; General transcription factor IIH subunit 2 antibody; AtGTF2H2 antibody; TFIIH basal transcription factor complex p44 subunit antibody; Atp44 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GTF2H2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. Can restore UV resistance in the NER-deficient ssl1-1 yeast mutant.
Gene References into Functions
  1. AtGTF2H2 can act in transcription and nucleotide excision repair(NER), and it participates in both processes in Arabidopsis as part of TFIIH. [AtGTF2H2] PMID: 16623910
Subcellular Location
Nucleus.
Protein Families
GTF2H2 family
Database Links

KEGG: ath:AT1G05055

STRING: 3702.AT1G05055.1

UniGene: At.43339

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