CDC34 Antibody

1. Cdc34 phosphorylation by PKA and Sch9 provides a direct tether between G1 cell division events and cell growth PMID: 22087249
2. Cdc34 showed a strong preference for Ub lysine 48 (K48), with lower activity towards lysine 11 (K11) and lysine 63 (K63). PMID: 23656784
3. chromosome segregation in cdc6 mutant is caused by the deregulation of spindle dynamics induced via a regulatory network involving the ubiquitin-conjugating enzyme Cdc34, microtubule-associated proteins and the anaphase-promoting complex activator Cdh1. PMID: 22805765
4. Cdc34 is particularly sensitive to oxidative inactivation, through sequestration of the catalytic cysteine in a disulfide complex with Uba1, by levels of oxidant that do not reduce global ubiquitinylation of proteins. PMID: 22949505
5. Through analysis of the phenotype of a mutant missing a highly conserved sequence motif within the catalytic domain of Cdc34, we discovered previously unrecognized levels of regulation PMID: 21196523
6. Data show that proximal amino acids surrounding the lysine residues in Sic1 and Ub are critical for ubiquitination, and that this mechanism is linked to key residues composing the catalytic core of Cdc34 and independent of SCF. PMID: 20194622
7. The acidic loop of CDC34 favorably positions K48 of a substrate-linked ubiquitin to attack SCF bound Cdc34-ubiquitin thioester and thereby enables processive synthesis of K48-linked ubiquitin chains by SCF-Cdc34. PMID: 16360039
8. We propose that interactions between Cdc34 approximately Ub and SCF directly activate ubiquitin transfer within a substrate-SCF-Cdc34 approximately Ub ternary complex. PMID: 17114057
9. studies demonstrate that CK2-mediated phosphorylation of Cdc34 on the acidic tail domain stimulates Cdc34-SCFCdc4 ubiquitination activity and cell cycle progression PMID: 17461777
10. In in vitro experiments with single-lysine-containing Cdc34 mutant proteins of Saccharomyces cerevisiae, the SCF-mediated stimulation of autoubiquitination is limited to specific N-terminal lysines modified via an intermolecular mechanism. PMID: 17562869
11. The CK2 phosphorylation of catalytic domain of Cdc34 modulates its activity at the G1 to S transition in Saccharomyces cerevisiae. PMID: 18418079
12. The acidic tail of the Cdc34 ubiquitin-conjugating enzyme functions in both binding to and catalysis with ubiquitin ligase SCFCdc4. PMID: 19875449

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KEGG: sce:YDR054C

STRING: 4932.YDR054C