porin Antibody

Code CSB-PA856714XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) porin Polyclonal antibody
Uniprot No.
Target Names
porin
Alternative Names
porin antibody; POR-1 antibody; VDAC antibody; CG6647Voltage-dependent anion-selective channel antibody; DmVDAC antibody; Porin antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) porin protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. In depolarized mitochondria, acts downstream of park to promote mitophagy or prevent apoptosis; polyubiquitination by park promotes mitophagy, while monoubiquitination by park decreases mitochondrial calcium influx which ultimately inhibits apoptosis.
Gene References into Functions
  1. The inhibition of porin phenocopies Parkinson disease phenotypes in Drosophila, while the overexpression of Buffy can counteract these phenotypes to improve the overall "healthspan" of the organism. PMID: 27881168
  2. Drosophila porin mutants have a role in mitochondrial dysfunction and disease PMID: 20110367
  3. identification of two PlacW insertions in the porin gene among a set of P-element insertions that have been mapped to the 32B3-4 region on the second chromosome PMID: 12207222
  4. Our data suggest that the PORIN protein is important in maintaining a photoreceptor response during prolonged stimulation. PMID: 17525991
  5. Porin isoform 1 is selectively targeted to follicular cells while Porin isoform 2 is present in mitochondria of the epithelial sheath cells of the ovariole. PMID: 18686020

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Subcellular Location
Mitochondrion outer membrane.
Protein Families
Eukaryotic mitochondrial porin family
Database Links

KEGG: dme:Dmel_CG6647

STRING: 7227.FBpp0079772

UniGene: Dm.33362

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