cry1Ia Antibody

Code CSB-PA673817ZA01BDC
Size US$299
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: cry1Ia antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 36.2 kDa
    Observed band size: 37 kDa
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Product Details

Uniprot No.
Target Names
cry1Ia
Alternative Names
Pesticidal crystal protein Cry1Ia (81 kDa crystal protein) (Crystaline entomocidal protoxin) (Insecticidal delta-endotoxin CryII(a)), cry1Ia, CGCryV cryII(a) cryV cryV1
Species Reactivity
Bacillus thuringiensis subsp. Kurstaki
Immunogen
Recombinant Bacillus thuringiensis subsp. Kurstaki Pesticidal crystal protein cry1Ia(cry1Ia),partial (97-180aa)
Immunogen Species
Bacillus thuringiensis subsp. Kurstaki
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

To create the cry1Ia antibody, a rabbit is immunized with recombinant Bacillus thuringiensis subsp. Kurstaki pesticidal crystal protein cry1Ia (97-180aa), leading to the production of specific IgG antibodies by the rabbit's B lymphocytes. The polyclonal cry1Ia antibody is then purified meticulously from the rabbit's serum utilizing protein G techniques. This cry1Ia antibody is recommended for the detection of Bacillus thuringiensis subsp. Kurstaki cry1Ia protein in both ELISA and WB assays.

Bacillus thuringiensis subsp. Kurstaki Cry1Ia mainly acts as a pesticidal toxin, targeting and affecting the larvae of certain insect pests, particularly those of the Lepidoptera order. It does so by binding to specific receptors in the insect's midgut, ultimately leading to gut damage, disruption of feeding, and, consequently, the death of the insect larvae.

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Target Background

Function
Promotes colloidosmotic lysis by binding to the midgut epithelial cells of certain coleopteran and lepidopteran species. Active on Plutella xylostella and Bombyx mori.
Protein Families
Delta endotoxin family
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