fimA Antibody

Datasheet

Code	CSB-PA361210ZA01ENV
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: fimA antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31.8 kDa Observed band size: 36 kDa
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) fimA Polyclonal antibody

Uniprot No.

P04128

Target Names

fimA

Alternative Names

fimA antibody; pilA antibody; b4314 antibody; JW4277Type-1 fimbrial protein antibody; A chain antibody; Type-1A pilin antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli

Immunogen

Recombinant Escherichia coli Type-1 fimbrial protein, A chain (fimA) (24-182AA)

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein A/G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

This rabbit polyclonal antibody against fimA is developed in a rabbit. The peptide sequence for the immunogen was taken from within the amino acids 24-182 of the recombinant Escherichia coli fimA protein. This fimA antibody is purified by protein A/G. Two applications ELISA and WB have been used in assays to test for the specificity of this fimA antibody. It can react with Escherichia coli fimA protein.

The fimA is the major structural subunit, which forms the majority of the Escherichia coli type-1 fimbrial. Studies have shown that type 1 fimbriae, and particularly fimA, as an abundant surface protein, are potent targets for host immunity. The fimA protein, along with other fimbrial proteins, allows Escherichia coli to adhere to specific receptors or ligands on host cells, tissues, or abiotic surfaces.

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Target Background

Function

Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.

Gene References into Functions

FimA is capable of intramolecular self-complementation via its own donor strand, as evidenced by the loss of folding competence upon donor strand deletion. PMID: 21816158
Results show that pilA recombinant protein has some immunoprotection effect with the challenging of virulent strains of E. coli GH1.2. PMID: 17577990
report the identification of a soluble form of the pilus protein FimA from the culture supernatants of E. coli K1, Salmonella, and Shigella that can potently inhibit Bax-mediated release of cytochrome c from isolated mitochondria. PMID: 20347420

Subcellular Location

Fimbrium.

Protein Families

Fimbrial protein family

Database Links

KEGG: ecj:JW4277

STRING: 316407.85677057

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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fimA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background