hlyE Antibody

Code CSB-PA303529ZA01ENV
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Product Details

Full Product Name Rabbit anti-Escherichia coli (strain K12) hlyE Polyclonal antibody
Uniprot No. P77335
Target Names hlyE
Alternative Names hlyE; clyA; hpr; sheA; ycgD; b1182; JW5181; Hemolysin E, chromosomal; Cytotoxin ClyA; Hemolysis-inducing protein; Latent pore-forming 34 kDa hemolysin; Silent hemolysin SheA
Raised in Rabbit
Species Reactivity Escherichia coli
Immunogen Recombinant Escherichia coli hlyE protein
Immunogen Species Escherichia coli (strain K12)
Conjugate Non-conjugate
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (12-14 weeks)

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Target Background

Function
(From Uniprot)
Toxin, which has some hemolytic activity towards mammalian cells. Acts by forming a pore-like structure upon contact with mammalian cells.
Gene References into Functions
  1. Membrane-triggered conformational transition of ClyA has been caprured and reported. PMID: 27797514
  2. The assembly dynamics of the cytolytic pore toxin ClyA has been reported. PMID: 25652783
  3. These findings show that the clyA+ locus is expressed at an elevated level in the uropathogenic Escherichia coli strain and the authors conclude that this is at least in part due to the effect of the SfaX/PapX transcriptional regulators. PMID: 25178918
  4. ClyA is delivered to the target host cell in an oligomeric conformation within outer membrane vesicles (OMVs), our findings suggest ClyA forms a prepore oligomeric structure independently of the lipid membrane within the OMV. PMID: 24019520
  5. Novel site(s) in HlyB responsible for enhancing secretion of cyclodextrin glucanotransferase in E. coli. PMID: 20959127
  6. in extraintestinal Escherichi coli infections, no HylaA+ sheA+ strains were identified, suggesting possible incompatibility between HlyA and SheA in the chromosome of E. coli PMID: 15956433
  7. analysis of the hemolysin E (HlyE, ClyA, and SheA) channel in its membrane-bound form PMID: 16754675
  8. Data Show that hlyE expression in Escherichia coli was controlled by alternate SlyA and H-NS nucleoprotein complexes. PMID: 17892462
  9. Circular dichroism and fluorescence energy transfer analyses show that HlyE protomers retain an alpha-helical structure when oligomerized to form a pore consisting of parallel HlyE protomers. PMID: 18227266
  10. results failed to show a positive correlation with SIDS, instead proving that clyA and irp2 genes were common to the infant intestinal E. coli PMID: 19208875

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Subcellular Location Secreted. Periplasm. Host cell membrane; Single-pass membrane protein. Note=Exported from the cell by outer membrane vesicles. Also found in the periplasmic space.
Protein Families Hemolysin E family
Database Links

KEGG: ecj:JW5181

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