ompA Antibody

Code CSB-PA359226ZA01EOD
Size US$299
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: ompA antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 35.6 kDa
    Observed band size: 36 kDa
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Product Details

Uniprot No.
Target Names
ompA
Alternative Names
Outer membrane protein A (Outer membrane protein II*), ompA
Species Reactivity
Escherichia coli O157:H7
Immunogen
Recombinant Escherichia coli O157:H7 ompA protein (164-346aa )
Immunogen Species
Escherichia coli O157:H7
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The ompA polyclonal antibody is produced by immunizing a rabbit with the recombinant Escherichia coli O157:H7 ompA protein (164-346aa). After collection from the rabbit serum, the ompA antibody is purified using protein G. This ompA antibody is specifically designed for detecting the Escherichia coli O157:H7 ompA protein in ELISA and WB experiments.

The OmpA protein of Escherichia coli O157:H7 is an outer membrane protein that plays a role in bacterial adhesion to host cells and in the formation of biofilms. It has also been implicated in the pathogenesis of E. coli O157:H7 infections, as it is involved in the modulation of host immune responses and the induction of apoptosis in host cells.

Customer Reviews and Q&A

 Customer Reviews
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Applications : Western blot analysis

Sample type: cells

Review: OmpA-expression of ATCC 17978 and the blsA mutant in response to blue light. The broad-range anti-GAPDH was used as a loading control. Statistical analyses were performed using Student’s t test (two-tailed) for comparisons of two groups (**, P , 0.01; ***, P , 0.001).

By Anonymous

Applications : Western Blot (WB)

Sample type: cell

Sample dilution: /

Review: I used CSB-PA359226ZA01EOD antibody to detect WB of bacterial extracellular vesicles secreted by E.coli Among different density gradient centrifugation fractions, OmpA protein was mainly enriched in 6-8 fractions, and the band position was mainly around 35 kDa.

By Anonymous

Target Background

Function
With TolR probably plays a role in maintaining the position of the peptidoglycan cell wall in the periplasm. Acts as a porin with low permeability that allows slow penetration of small solutes; an internal gate slows down solute passage.; Required for conjugation with F-type plasmids; probably serves as the mating receptor on recipient cells.
Gene References into Functions
  1. C-terminal domain of OmpA is responsible for bacterial resistance to acidic conditions, high osmotic pressure and high SDS. environments PMID: 26686619
  2. OMPA is a modulator of Escherichia coli O157:H7 adherence to bovine rectoanal junction squamous epithelial cells. PMID: 25643951
Subcellular Location
Cell outer membrane; Multi-pass membrane protein.
Protein Families
OmpA family
Database Links

KEGG: ece:Z1307

STRING: 155864.Z1307

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