pykF Antibody

Code CSB-PA364920ZA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) pykF Polyclonal antibody
Uniprot No.
Target Names
pykF
Alternative Names
Pyruvate kinase I (EC 2.7.1.40) (PK-1), pykF
Raised in
Rabbit
Species Reactivity
Escherichia coli
Immunogen
Recombinant Escherichia coli pykF protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugate
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Catalyzes the formation of pyruvate in the last step of glycolysis, it is irreversible under physiological conditions. The reaction is critical for the control of metabolic flux in the second part of glycolysis.
Gene References into Functions
  1. data are consistent with the domain rotation model but inconsistent with the rigid body reorientation model given the increased flexibility at the interdomain interface, and we can for the first time explain how fructose 1,6-bisphosphate affects the active site. PMID: 26879751
  2. A pykF-gene knockout mutation effects gene expression and metabolic fluxes in Escherichia coli. PMID: 15158258
  3. The elevated cAMP in PB25 suggests that pyruvate kinase may participate in glucose catabolite repression by serving among all of the factors that tighten gene expression. PMID: 19251844
Protein Families
Pyruvate kinase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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