Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

pykF

Uniprot No.

P0AD61

Research Area

Metabolism

Alternative Names

PK-1

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

1-470aa

Target Protein Sequence

MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHGQRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGNSEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNLPGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHIISKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICERTDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFPDATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVVMVSGALVPSGTTNTASVHVL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

54.8 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-470 constitute the expression domain of recombinant Escherichia coli (strain K12) pykF. This pykF protein is theoretically predicted to have a molecular weight of 54.8 kDa. Expression of this pykF protein is conducted in e.coli. The pykF gene fragment has been modified by fusing the N-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant pykF protein during the following stages.

Escherichia coli pyruvate kinase I (PykF) is a crucial enzyme in glycolysis, catalyzing the conversion of phosphoenolpyruvate to pyruvate and producing ATP. Its significance extends across diverse research domains. In metabolic engineering, PykF enhances compound production by influencing glycolytic pathways. Biotechnological applications leverage its role in cellular energy metabolism for optimizing processes in industrial settings. As a potential target for antibiotic development, inhibiting PykF could disrupt bacterial metabolism. Studies of PykF contribute to understanding bacterial physiology, adaptive responses to nutrient variations, and the broader landscape of functional genomics.

Target Background

Function

Catalyzes the formation of pyruvate in the last step of glycolysis, it is irreversible under physiological conditions. The reaction is critical for the control of metabolic flux in the second part of glycolysis.

Gene References into Functions

data are consistent with the domain rotation model but inconsistent with the rigid body reorientation model given the increased flexibility at the interdomain interface, and we can for the first time explain how fructose 1,6-bisphosphate affects the active site. PMID: 26879751
A pykF-gene knockout mutation effects gene expression and metabolic fluxes in Escherichia coli. PMID: 15158258
The elevated cAMP in PB25 suggests that pyruvate kinase may participate in glucose catabolite repression by serving among all of the factors that tighten gene expression. PMID: 19251844

Protein Families

Pyruvate kinase family

Database Links

KEGG: ecj:JW1666

STRING: 316385.ECDH10B_1810

Code	CSB-EP364920ENV
Abbreviation	Recombinant E.coli pykF protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Escherichia coli Pyruvate kinase I (pykF)

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