Product Details

Full Product Name

Rabbit anti-Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) rhlR Polyclonal antibody

Uniprot No.

P54292

Target Names

rhlR

Alternative Names

rhlR antibody; lasM antibody; vsmR antibody; PA3477 antibody; Regulatory protein RhlR antibody; Elastase modulator antibody

Raised in

Rabbit

Species Reactivity

Pseudomonas aeruginosa

Immunogen

Recombinant Pseudomonas aeruginosa rhlR protein

Immunogen Species

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (12-14 weeks)

Target Background

Function

Necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase. It also functions as a transcriptional activator of elastase structural gene (lasB). Binds to autoinducer molecules BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone).

Gene References into Functions

this work shows that PqsE and RhlR function as a QS-autoinducer synthase-receptor pair that drives group behaviors in P. aeruginosa. PMID: 30224496
rhlR/lasR knockout increased the susceptibility of Pseudomonas aeruginosa towards polymyxin B-azithromycin combination via influencing quorum sensing. PMID: 28096154
This is the first time that a domain of the quorum sensing regulator RhlR was produced in sufficient amounts for structural studies, enabling the investigation of the molecular basis for RhlR specific interaction with DNA promoters. PMID: 26792557
Quorum sensing genes lasR and rhlR and activity of electric field sensitive enzyme, glycerol-3-phosphate dehydrogenase was also repressed by WED. PMID: 25803639
Subinhibitory concentrations of macrolides specifically upregulated expression of quorum sensing genes (rhlR, lasI), whereas sulfonamides and municipal wastewater, instead upregulated expression of specific resistant genes (sul1) and efflux pumps (mexD). PMID: 23392972
The rmlBDAC operon has three promoters, and that the expression of the P2 promoter is dependent on the stationary phase sigma factor sigmaS and on the quorum sensing regulator RhlR. PMID: 22262098
rhlR transcription is subject to positive-feedback autoregulation through RhlR/C4-HSL activation of the rhlA promoter. PMID: 21719541
RhlR and LasR regulate the anthranilate metabolism in a mutually antagonistic and growth phase-differential manner. PMID: 21614486
Analysis of rpoS gene and the rhlR gene per cell showed that P. aeruginosa cells express different proteins according to their location in the biofilm. PMID: 20348255
MvfR contributes to P. aeruginosa virulence by controlling the transcription of genes not under RhlR regulation. PMID: 15686549
down-regulation of the T3S regulon in the presence of RhlR-C4HSL and the corresponding advanced secretion of ExoS in a rhlI mutant. PMID: 15901720
Data found that lon-disrupted cells show increased levels of a transcriptional regulator, RhlR. PMID: 18408026
RhlR is able to induce LasR-regulated genes in the absence of lasR. PMID: 19246742

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Protein Families

Autoinducer-regulated transcriptional regulatory protein family

Database Links

KEGG: pae:PA3477

STRING: 208964.PA3477

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rhlR Antibody

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