Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

rhlR

Uniprot No.

P54292

Research Area

others

Alternative Names

rhlR; lasM; vsmR; PA3477; Regulatory protein RhlR; Elastase modulator

Species

Pseudomonas aeruginosa

Source

E.coli

Expression Region

1-241aa

Target Protein Sequence

MRNDGGFLLWWDGLRSEMQPIHDSQGVFAVLEKEVRRLGFDYYAYGVRHTIPFTRPKTEVHGTYPKAWLERYQMQNYGAVDPAILNGLRSSEMVVWSDSLFDQSRMLWNEARDWGLCVGATLPIRAPNNLLSVLSVARDQQNISSFEREEIRLRLRCMIELLTQKLTDLEHPMLMSNPVCLSHREREILQWTADGKSSGEIAIILSISESTVNFHHKNIQKKFDAPNKTLAAAYAAALGLI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

47.6kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The DNA fragment encoding the 1-241aa of the Pseudomonas aeruginosa rhlR protein was fused with N-terminal 10xHis-SUMO tag and C-terminal Myc tag gene and then was inserted into the expression vector, which was subsequently transformed into the E.coli for expression. The resulting product was further purified to obtain the recombinant Pseudomonas aeruginosa rhlR protein. The purity of this recombinant rhlR protein is greater than 90% assessed by Bandscan software analysis combined with SDS-PAGE. This recombinant rhlR protein showed a band on the gel with a molecular weight of approximately 45 kDa.

rhlR is a gene encoding a protein named regulatory protein RhlR and belongs autoinducer-regulated transcriptional regulatory protein family.This protein is necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase. Regulation of gene expression plays a key role in bacterial adaptability to changes in the environment. In P. aeruginosa,quorum sensing (QS) relies on the activation of specific transcriptional regulators (LasR and RhlR) by corresponding signals synthesized by their cognate acyl HSL synthases (LasI and RhlI). rhlR transcription is subject to positive-feedback autoregulation through RhlR/C4-HSL activation of the rhlA promoter.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

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Applications : Binding assay

Review: The RhlR protein can bind to the promoter region of the tesG gene as determined by EMSA. Images are representative of three independent replicates.

By Anonymous

Code	CSB-EP346531EZX
Abbreviation	Recombinant Pseudomonas aeruginosa rhlR protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP346531EZX could indicate that this peptide derived from E.coli-expressed Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) rhlR. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP346531EZX could indicate that this peptide derived from E.coli-expressed Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) rhlR.
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Recombinant Pseudomonas aeruginosa Regulatory protein RhlR (rhlR)

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