Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
rhlR; lasM; vsmR; PA3477; Regulatory protein RhlR; Elastase modulator
Species
Pseudomonas aeruginosa
Expression Region
1-241aa
Target Protein Sequence
MRNDGGFLLWWDGLRSEMQPIHDSQGVFAVLEKEVRRLGFDYYAYGVRHTIPFTRPKTEVHGTYPKAWLERYQMQNYGAVDPAILNGLRSSEMVVWSDSLFDQSRMLWNEARDWGLCVGATLPIRAPNNLLSVLSVARDQQNISSFEREEIRLRLRCMIELLTQKLTDLEHPMLMSNPVCLSHREREILQWTADGKSSGEIAIILSISESTVNFHHKNIQKKFDAPNKTLAAAYAAALGLI
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The DNA fragment encoding the 1-241aa of the Pseudomonas aeruginosa rhlR protein was fused with N-terminal 10xHis-SUMO tag and C-terminal Myc tag gene and then was inserted into the expression vector, which was subsequently transformed into the E.coli for expression. The resulting product was further purified to obtain the recombinant Pseudomonas aeruginosa rhlR protein. The purity of this recombinant rhlR protein is greater than 90% assessed by Bandscan software analysis combined with SDS-PAGE. This recombinant rhlR protein showed a band on the gel with a molecular weight of approximately 45 kDa.
rhlR is a gene encoding a protein named regulatory protein RhlR and belongs autoinducer-regulated transcriptional regulatory protein family.This protein is necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase. Regulation of gene expression plays a key role in bacterial adaptability to changes in the environment. In P. aeruginosa,quorum sensing (QS) relies on the activation of specific transcriptional regulators (LasR and RhlR) by corresponding signals synthesized by their cognate acyl HSL synthases (LasI and RhlI). rhlR transcription is subject to positive-feedback autoregulation through RhlR/C4-HSL activation of the rhlA promoter.
