Bovine Epithelial growth factor(EGF)ELISA Kit

Code CSB-E17171B
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation EGF
Target Name Epithelial growth factor(EGF)
Alias HOMG4, URG, beta-urogastrone|epidermal growth factor
Species Bos taurus (Bovine)
Sample Types serum, plasma
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
HGNC 1850
RGD 1560078
MGI 1100883
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %88-97
1:2Average %106
Range %100-109
1:4Average %90
Range %85-94
1:8Average %94
Range %90-97
Recovery
The recovery of bovine EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9387-97
EDTA plasma (n=4)9489-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.109 2.042 2.076 1.952
51.710 1.723 1.717 1.593
2.51.432 1.421 1.427 1.303
1.251.018 0.999 1.009 0.885
0.6250.678 0.668 0.673 0.549
0.3120.466 0.451 0.459 0.335
0.1560.299 0.292 0.296 0.172
00.125 0.123 0.124
References
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