Bovine Heat shock protein HSP 90-alpha(HSP90AA1) ELISA kit

Instructions
Code CSB-EL010802BO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Alternative Names HSP90AA1 ELISA Kit; HSP90A ELISA Kit; HSPCA ELISA Kit; Heat shock protein HSP 90-alpha ELISA Kit
Abbreviation HSP90AA1
Uniprot No. Q76LV2
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine HSP90AA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %86-95
1:2Average %102
Range %97-107
1:4Average %91
Range %85-97
1:8Average %97
Range %91-103
Recovery
The recovery of bovine HSP90AA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)9588-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
4002.192 2.178 2.185 2.029
2001.684 1.656 1.670 1.514
1001.107 1.121 1.114 0.958
500.651 0.637 0.644 0.488
250.462 0.482 0.472 0.316
12.50.328 0.331 0.330 0.174
6.250.241 0.251 0.246 0.090
00.155 0.157 0.156
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from Hsp90 which acquires an open conformation for the next cycle. Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation. Interacts with HECTD1 (via N-terminus) (By similarity).
Gene References into Functions
  1. Data suggest that elevated temperatures stimulate PGE2 production in ampullary oviduct by increasing expression of HSP90AA1 and PGES (prostaglandin-E synthase). PMID: 23704311
  2. This study showed that ubiquitinated ALK5 and phosphorylated heat shock protein 27 specifically accumulate in the cytoskeleton fraction, and ALK1 and ALK5 interact with heat shock protein 90(HSP90). PMID: 21465483
Subcellular Location Nucleus, Cytoplasm, Melanosome, Cell membrane
Protein Families Heat shock protein 90 family
Database Links

KEGG: bta:281832

STRING: 9913.ENSBTAP00000008225

UniGene: Bt.23179

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