Bovine Immunoglobulin G,IgG ELISA Kit

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Code CSB-E12015B
Size 96T,5×96T,10×96T
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Product Details

Abbreviation
IgG
Species
Bos taurus (Bovine)
Sample Types
serum, plasma
Detection Range
78 ng/ml-5000ng/ml
Sensitivity
78 ng/ml
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Sample

Serum(n=4)

1:100

Average %

89

Range %

85-92

1:200

Average %

97

Range %

92-101

1:400

Average %

91

Range %

87-94

1:800

Average %

95

Range %

91-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

5000

0.099

0.101

0.100

2500

0.179

0.171

0.175

1250

0.291

0.286

0.289

625

0.500

0.482

0.491

312

0.936

0.917

0.927

156

1.281

1.223

1.252

78

1.967

1.986

1.977

0

2.673

2.758

2.716

Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

The Bovine immunoglobulin G (IgG) ELISA Kit facilitates quantitative measurement of bovine IgG in serum and plasma. It features a detection range of 78 ng/mL-5000 ng/mL and a sensitivity of 19.5 ng/mL. Using a competitive detection mechanism, the kit involves the IgG in the sample competing with a labeled IgG for binding to a limited number of antibody sites. This competition results in an inverse relationship between the IgG concentration in the sample and the signal intensity, allowing for precise quantification. The kit requires 50-100 μL of sample and 1-5 hours of assay time, with results detected at a wavelength of 450 nm. More than eight academic papers have utilized this kit.

Citations

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