This human IgG ELISA Kit is suitable for qualitatively determining human IgG concentrations in serum, plasma, cell culture supernates, urine, tissue homogenates, or cell lysates in vitro. IgG exists in mucosal secretions in the nose and along the tracheobronchial tree. It is the most common form of antibody found in blood and extracellular fluid and helps the body fight infection. IgG functions to increase the phagocytosis of pathogens, neutralize bacterial or viral toxins and trigger the activation of the complement system. By binding many kinds of pathogens such as viruses, bacteria, and fungi, IgG protects the body from infection.
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with human IgG. Standards and samples are pipetted into the wells with an HRP-conjugated human IgG antibody. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops oppositely to the amount of human IgG in samples. The color development is stopped and the intensity of the color is measured. Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.