Human Immunoglobulin G(IgG)ELISA Kit

Code CSB-E07979h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This human IgG ELISA Kit is suitable for qualitatively determining human IgG concentrations in serum, plasma, cell culture supernates, urine, tissue homogenates, or cell lysates in vitro. IgG exists in mucosal secretions in the nose and along the tracheobronchial tree. It is the most common form of antibody found in blood and extracellular fluid and helps the body fight infection. IgG functions to increase the phagocytosis of pathogens, neutralize bacterial or viral toxins and trigger the activation of the complement system. By binding many kinds of pathogens such as viruses, bacteria, and fungi, IgG protects the body from infection.

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with human IgG. Standards and samples are pipetted into the wells with an HRP-conjugated human IgG antibody. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops oppositely to the amount of human IgG in samples. The color development is stopped and the intensity of the color is measured. Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

Target Name Immunoglobulin G(IgG)
Alternative Names N/A
Abbreviation IgG
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, urine, tissue homogenates, cell lysates
Detection Range 0.59 μg/mL-150 μg/mL
Sensitivity 0.487 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision

 

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

 

Linearity

 

To assess the linearity of the assay, samples were spiked with high concentrations of human IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

 

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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