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Pig Immunoglobulin G(IgG) ELISA Kit

Citations (16) Protocol
Code CSB-E06804p
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
Immunoglobulin G(IgG)
Alternative Names
N/A
Abbreviation
IgG
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.586 μg/mL-150 μg/mL
Sensitivity
0.467 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:200 Average % 90  
Range % 85-96  
1:400 Average % 95  
Range % 91-107  
1:800 Average % 93  
Range % 86-99  
1:1600 Average % 91  
Range % 88-97  
Recovery
The recovery of pig IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-98  
EDTA plasma (n=4) 94 89-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
150 0.254 0.251 0.253    
37.5 0.555 0.517 0.536    
9.38 0.755 0.721 0.738    
2.34 0.999 0.981 0.990    
0.58 1.169 1.113 1.141    
0 1.481 1.465 1.473    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This pig arachidonic acid (AA) ELISA Kit is suitable for qualitatively determining pigarachidonic acid concentrations in serum, plasma, cell culture supernates, or tissue homogenates in vitro. IgG is the major class of immunoglobulins found in blood and extracellular fluid, allowing itself to control infection of body tissues. It is the exclusive isotype that is transferred across the placenta. In the process of protecting the body against virus invasion, IgG mainly exerts its functions through several mechanisms: directly binding to and neutralizing the virus, preventing it from invading cells; IgG binds to the surface antigen of the infected cell and labeled it, and then recruit phagocytes to clear the virus; IgG triggers an immune response by activating complement and binding immune cells; IgG can also pass through the placental barrier and be passively transmitted from the mother to the fetus, achieving natural passiveness.

This assay employs the competitive inhibition enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops oppositely to the amount of pig IgG in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including sensitivity, specificity, precision, linearity, and recovery.

Citations

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Customer Reviews and Q&A

■ Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Sample type: Serum

Sample species: Pig

Sample dilution: No dilution

Review: The calibration is accurate, and the sample values are within the calibration range, which is in line with the expected results.

By Anonymous

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