Bovine Interleukin 12,IL-12 ELISA Kit

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Bovine Interleukin 12,IL-12 ELISA Kit

Name: Bovine Interleukin 12,IL-12 ELISA Kit
Brand: CUSABIO
SKU: CSB-E14010B

^Instructions

Code	CSB-E14010B
Size	96T,5×96T,10×96T
Trial Size
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Target Data

Target Name

interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)

Alternative Names

IL12AInterleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit

Abbreviation

IL12A

Uniprot No.

P54349

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, tissue homogenates

Detection Range

6.25 pg/mL-400 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	98
	Range %	91-102
1:4	Average %	94
	Range %	90-98
1:8	Average %	94
	Range %	86-99

Recovery

The recovery of bovine IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-92
EDTA plasma (n=4)	97	95-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.758	2.628	2.693	2.531
200	2.184	2.084	2.134	1.972
100	1.612	1.562	1.587	1.425
50	1.203	1.173	1.188	1.026
25	0.652	0.642	0.647	0.485
12.5	0.384	0.374	0.379	0.217
6.25	0.258	0.253	0.256	0.094
0	0.163	0.161	0.162

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Evaluation of the immune responses against reduced doses of Brucella abortus S19 (calfhood) vaccine in water buffaloes (Bubalus bubalis), India R Shome,Vaccine,2020

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Function	Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Gene References into Functions	The results showed that the expression of TNF-alpha, iNOS, and IL-6 in alveolar macrophages was up-regulated by stimulation with the recombinant Mce4A protein of M. bovis; in contrast, expression of IL-12 was unaffected.[IL-6, IL-12] PMID: 17530193 Lipopolysaccharide stimulation up-regulates the secretion of cytokines, including interleukin-12, by bovine polymorphonuclear neutrophil leukocytes. PMID: 17727806
Subcellular Location	Secreted
Protein Families	IL-6 superfamily
Database Links	KEGG: bta:281856 STRING: 9913.ENSBTAP00000020153 UniGene: Bt.524

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Related pathways

Jak-STAT signaling pathway Th1 and Th2 cell differentiation Toll-like receptor signaling pathway

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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