Target Name
interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
Alternative Names
IL12AInterleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 80-92 | |
| 1:2 | Average % | 98 | |
| Range % | 91-102 | |
| 1:4 | Average % | 94 | |
| Range % | 90-98 | |
| 1:8 | Average % | 94 | |
| Range % | 86-99 | |
Recovery
| The recovery of bovine IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 84-92 | |
| EDTA plasma (n=4) | 97 | 95-101 | |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.758 | 2.628 | 2.693 | 2.531 | | | 200 | 2.184 | 2.084 | 2.134 | 1.972 | | | 100 | 1.612 | 1.562 | 1.587 | 1.425 | | | 50 | 1.203 | 1.173 | 1.188 | 1.026 | | | 25 | 0.652 | 0.642 | 0.647 | 0.485 | | | 12.5 | 0.384 | 0.374 | 0.379 | 0.217 | | | 6.25 | 0.258 | 0.253 | 0.256 | 0.094 | | | 0 | 0.163 | 0.161 | 0.162 | | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Bovine IL12A ELISA Kit was designed for the quantitative measurement of Bovine IL12A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .
