Bovine Interleukin 12,IL-12 ELISA Kit

Code CSB-E14010B
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
Promotion 25% Off Above 3 its (96T) Per Item
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name
interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
Alternative Names
IL12AInterleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
Uniprot No.
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
1.56 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %86
Range %80-92
1:2Average %98
Range %91-102
1:4Average %94
Range %90-98
1:8Average %94
Range %86-99
The recovery of bovine IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8984-92
EDTA plasma (n=4)9795-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
4002.758 2.628 2.693 2.531
2002.184 2.084 2.134 1.972
1001.612 1.562 1.587 1.425
501.203 1.173 1.188 1.026
250.652 0.642 0.647 0.485
12.50.384 0.374 0.379 0.217
6.250.258 0.253 0.256 0.094
00.163 0.161 0.162  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Bovine IL12A ELISA Kit was designed for the quantitative measurement of Bovine IL12A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

(From Uniprot)
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Gene References into Functions
  1. The results showed that the expression of TNF-alpha, iNOS, and IL-6 in alveolar macrophages was up-regulated by stimulation with the recombinant Mce4A protein of M. bovis; in contrast, expression of IL-12 was unaffected.[IL-6, IL-12] PMID: 17530193
  2. Lipopolysaccharide stimulation up-regulates the secretion of cytokines, including interleukin-12, by bovine polymorphonuclear neutrophil leukocytes. PMID: 17727806
Subcellular Location
Protein Families
IL-6 superfamily
Database Links

KEGG: bta:281856

STRING: 9913.ENSBTAP00000020153

UniGene: Bt.524

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
7505 Fannin St Ste 610-322 Houston, TX 77054, USA
Join Us with

Leave a message

check code
© 2007-2023 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1