Bovine interleukin 4,IL-4 ELISA Kit

Code CSB-E12898B
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Bovine IL4 ELISA Kit was designed for the quantitative measurement of Bovine IL4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL .

Target Name interleukin 4
Alternative Names IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
Abbreviation IL4
Uniprot No. P30367
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 89-99  
1:2 Average % 97  
Range % 92-103  
1:4 Average % 90  
Range % 85-94  
1:8 Average % 84  
Range % 80-88  
Recovery
The recovery of bovine IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
EDTA plasma (n=4) 89 84-93  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
800 1.993 1.912 1.953 1.835  
400 1.576 1.616 1.596 1.478  
200 1.304 1.354 1.329 1.211  
100 0.939 0.962 0.951 0.833  
50 0.624 0.641 0.633 0.515  
25 0.441 0.419 0.430 0.312  
12.5 0.283 0.279 0.281 0.163  
0 0.119 0.117 0.118    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
Gene References into Functions
  1. These results support pregnancy as type 2 immune response biased, with increases of IFN-gamma occurring after parturition and an increase in IL-4 production before calving. PMID: 23814356
  2. IL-4 may play a role in coordinating the adrenal response to inflammatory stress. PMID: 18055157
  3. T helper (Th) type 2 cell cytokine IL-4 modulates airway contraction by secreting matrix metalloproteinase-1 from the smooth muscle cells via phosphatidylinositol 3-kinase activation and changing cell-to-matrix interactions. PMID: 18322231
Subcellular Location Secreted.
Protein Families IL-4/IL-13 family
Database Links

KEGG: bta:280824

STRING: 9913.ENSBTAP00000021215

UniGene: Bt.423

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