Canine Prostaglandin E2,PG-E2 ELISA Kit

Instructions
Code CSB-E13493c
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Prostaglandin E2,PG-E2
Abbreviation PG-E2
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 10 pg/mL-2000 pg/mL
Sensitivity 5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 90-104  
1:2 Average % 95  
Range % 89-105  
1:4 Average % 93  
Range % 87-100  
1:8 Average % 95  
Range % 90-101  
Recovery
The recovery of canine PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 85-96  
EDTA plasma (n=4) 96 89-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
2000 0.187 0.174 0.181    
500 0.320 0.315 0.318    
160 0.517 0.547 0.532    
40 0.814 0.848 0.831    
10 1.316 1.374 1.345    
0 1.958 1.933 1.946    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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