Rat Prostaglandin E2,PG-E2 ELISA Kit

Instructions
Code CSB-E07967r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Prostaglandin E2,PG-E2
Abbreviation PG-E2
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.5 pg/mL-100 pg/mL
Sensitivity 0.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 91-99  
1:2 Average % 92  
Range % 87-96  
1:4 Average % 93  
Range % 89-98  
1:8 Average % 105  
Range % 99-109  
Recovery
The recovery of rat PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-99  
EDTA plasma (n=4) 101 96-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
100 0.228 0.224 0.226    
25 0.365 0.348 0.357    
8 0.560 0.539 0.550    
2 0.813 0.787 0.800    
0.5 1.258 1.234 1.246    
0 2.031 2.176 2.104    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Q&A and Customer Reviews

 Customer Reviews
  • Sample type: Tissue homogenate (Brain)
    Sample species: Mouse
    Review: One model group differed from the control group, the other two did not. The operation steps of the product are still very simple, and the standards are also prepared with different concentrations.

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