Human Prostaglandin E2,PG-E2 ELISA Kit

Instructions
Code CSB-E07965h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Prostaglandin E2,PG-E2
Abbreviation PG-E2
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.5 pg/mL-300 pg/mL
Sensitivity 0.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 91-99  
1:2 Average % 92  
Range % 86-96  
1:4 Average % 97  
Range % 92-103  
1:8 Average % 84  
Range % 80-88  
Recovery
The recovery of human PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-98  
EDTA plasma (n=4) 90 83-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
300 0.193 0.201 0.197    
75 0.349 0.327 0.338    
24 0.523 0.531 0.527    
6 0.903 0.962 0.933    
1.5 1.316 1.393 1.355    
0 2.271 2.212 2.242    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

Can this kit test cell supernatnat?

A:
Thanks for your inquiry. Usually we suggest you test the sample types mentioned in the kit manual. If you want to test cell supernatnat samples, you can do a pretest first.
 Customer Reviews

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