Code | CSB-E08550Ch |
Size | 96T,5×96T,10×96T |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 96 | ||||
Range % | 91-100 | |||||
1:2 | Average % | 92 | ||||
Range % | 88-97 | |||||
1:4 | Average % | 102 | ||||
Range % | 98-106 | |||||
1:8 | Average % | 94 | ||||
Range % | 90-98 |
The recovery of chicken IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 97 | 93-101 | ||||
EDTA plasma (n=4) | 92 | 88-96 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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This Chicken IFNG ELISA Kit was designed for the quantitative measurement of Chicken IFNG protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.125 pg/mL-800 pg/mL and the sensitivity is 3.125 pg/mL.
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Do you know if the chicken IFN-gamma kit shows cross-reactivity with other species?
There is no cross-reactivity with human, rat, and mouse recombinant proteins.