Recombinant Chicken Interferon gamma (IFNG)

The Recombinant Chicken IFNG protein is a protein encoded by recombinant DNA that was cloned in an expression vector that supported the expression of IFNG gene. This recombinant IFNG protein was expressed in the host. The expression region is 20-164aa of the Chicken IFNG. In the production, the expression vector contains N-terminal GST tag. Every production step was performed with a strict QC system. The purity of this protein is 90%+ determined by SDS-PAGE.

interferon-gamma/Interferon γ coded by the IFNG gene is among the common cytokines known to affect immune related disorders. Blockade of PD1/PD-L1 activates T cells accompanied by interferon- gamma (IFN-g) secretion, which has been proposed to mediate both pro- and anti-tumoral activities. IFN-g mediates adaptive resistance through PD-L1 dependent and independent mechanisms, which is becoming a key issue to broaden cancer immunotherapy. Other research demonstrates that liver type 1 innate lymphoid cells develop locally via an interferon-γ–dependent loop. Specifically, deficiency in the gene encoding interferon-γ or one of its receptors (Ifngr1) selectively reduced the frequency and number of ILC1s and not cNK cells in the liver. Delivery of a plasmid containing the interferon-γ (IFN-γ) cDNA to IFN-g-deficient mice via hydrodynamic tail-vein injection selectively increased the frequency and number of liver’s ILC1s but not of liver cNK cells. Moreover, IFN-γ signaling promoted the expansion and differentiation of LSM cells but not of ILC1s, supporting a model in which IFN-γ acts on these local progenitors to promote liver ILC1 development. The findings advance researchers’ knowledge of the importance of extramedullary hematopoiesis to cells of lymphoid origin.