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Chicken Interleukin 18,IL-18 ELISA KIT

Citations (3) Protocol
Code CSB-E10070Ch
Size 96T,5×96T,10×96T
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Product Details

Alternative Names
IL18 ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit
Abbreviation
IL18
Uniprot No.
Q8QFQ8
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, tissue Homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 89-102  
1:2 Average % 90  
Range % 82-99  
1:4 Average % 93  
Range % 85-102  
1:8 Average % 96  
Range % 92-100  
Recovery
The recovery of chicken IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 81-99  
EDTA plasma (n=4) 95 89-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 1.981 1.927 1.954 1.851  
250 1.334 1.374 1.354 1.251  
125 0.920 0.915 0.918 0.815  
62.5 0.619 0.643 0.631 0.528  
31.2 0.395 0.376 0.386 0.283  
15.6 0.290 0.306 0.298 0.195  
7.8 0.211 0.224 0.218 0.115  
0 0.102 0.103 0.103    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

The Chicken Interleukin 18 (IL-18) ELISA KIT is a perfect tool for researchers who are interested in quantifying IL-18 levels in chicken samples,in the field of immunology,inflammatory and infection.This kit is designed to detect and quantify IL-18 in serum, plasma, and tissue homogenates of Gallus gallus (Chicken) samples.

With a detection range of 20 pg/mL-500 pg/mL and a sensitivity of 1.95 pg/mL, this ELISA kit offers high precision and accuracy in measuring IL-18 levels in your samples. The assay time takes only 1-5 hours, and the sample volume required is 50-100ul. The detection wavelength for this ELISA kit is 450 nm, making it a highly sensitive and specific assay. Its sandwich assay principle ensures that you get quantitative measurements with high reproducibility and reliability.

Citations

Related Products

IL18 Proteins

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Target Background

Function
(From Uniprot)
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Subcellular Location
Cytoplasm. Secreted.
Protein Families
IL-1 family
Database Links
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