Chicken Interleukin 18,IL-18 ELISA KIT

Code CSB-E10070Ch
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
interleukin 18 (interferon-gamma-inducing factor)
Alternative Names
IL18 ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit
Abbreviation
IL18
Uniprot No.
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, tissue Homogenates
Detection Range
20 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %89-102
1:2Average %90
Range %82-99
1:4Average %93
Range %85-102
1:8Average %96
Range %92-100
Recovery
The recovery of chicken IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9581-99
EDTA plasma (n=4)9589-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5001.981 1.927 1.954 1.851
2501.334 1.374 1.354 1.251
1250.920 0.915 0.918 0.815
62.50.619 0.643 0.631 0.528
31.20.395 0.376 0.386 0.283
15.60.290 0.306 0.298 0.195
7.80.211 0.224 0.218 0.115
00.102 0.103 0.103
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Chicken Interleukin 18 (IL-18) ELISA KIT is a perfect tool for researchers who are interested in quantifying IL-18 levels in chicken samples,in the field of immunology,inflammatory and infection.This kit is designed to detect and quantify IL-18 in serum, plasma, and tissue homogenates of Gallus gallus (Chicken) samples.

With a detection range of 20 pg/mL-500 pg/mL and a sensitivity of 1.95 pg/mL, this ELISA kit offers high precision and accuracy in measuring IL-18 levels in your samples. The assay time takes only 1-5 hours, and the sample volume required is 50-100ul. The detection wavelength for this ELISA kit is 450 nm, making it a highly sensitive and specific assay. Its sandwich assay principle ensures that you get quantitative measurements with high reproducibility and reliability.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Subcellular Location
Cytoplasm. Secreted.
Protein Families
IL-1 family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1