Chicken Interleukin 18,IL-18 ELISA KIT

Instructions
Code CSB-E10070Ch
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 18 (interferon-gamma-inducing factor)
Alternative Names IL18 ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit
Abbreviation IL18
Uniprot No. Q8QFQ8
Species Gallus gallus (Chicken)
Sample Types serum, plasma, tissue Homogenates
Detection Range 20 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %89-102
1:2Average %90
Range %82-99
1:4Average %93
Range %85-102
1:8Average %96
Range %92-100
Recovery
The recovery of chicken IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9581-99
EDTA plasma (n=4)9589-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5001.981 1.927 1.954 1.851
2501.334 1.374 1.354 1.251
1250.920 0.915 0.918 0.815
62.50.619 0.643 0.631 0.528
31.20.395 0.376 0.386 0.283
15.60.290 0.306 0.298 0.195
7.80.211 0.224 0.218 0.115
00.102 0.103 0.103
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Subcellular Location Secreted
Protein Families IL-1 family
Database Links

STRING: 9031.ENSGALP00000012772

UniGene: Gga.17593

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