Dog Interferon γ,IFN-γ ELISA Kit

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Dog Interferon γ,IFN-γ ELISA Kit

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Protocol

Code	CSB-E11262c-IS
Size	96T,5×96T,10×96T
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Product Details
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Customer Reviews and Q&A
Target Background

Target Name

interferon, gamma

Associated names

IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit

Abbreviation

IFNG

Uniprot No.

P42161

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Types

serum, plasma, tissue homogenates

Detection Range

3.12pg/mL-200pg/mL

Sensitivity

1.56pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-95
1:2	Average %	100
	Range %	96-106
1:4	Average %	93
	Range %	89-98
1:8	Average %	106
	Range %	101-111

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
200	3.403	3.234	3.319	3.140
100	2.580	2.427	2.504	2.325
50	1.355	1.451	1.403	1.224
25	0.834	0.829	0.832	0.653
12.5	0.527	0.501	0.514	0.335
6.25	0.329	0.343	0.336	0.157
3.12	0.255	0.244	0.249	0.071
0	0.183	0.175	0.179

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days

Description

This ELISA kit provides quantitative measurement of interferon-gamma (IFNG) in canine samples, supporting immunological research where this pivotal Th1 cytokine drives cell-mediated immune responses in dogs. Because IFNG is often present at low baseline concentrations and rises sharply during infection or immune activation, accurate quantification across both resting and stimulated states is essential.

Detection Range: The 3.12–200 pg/mL range spans the concentrations typically observed from baseline circulating IFNG in healthy dogs through the elevated levels seen during active infections such as leishmaniasis or ehrlichiosis, minimizing the need for extensive sample dilution in most disease-monitoring studies.

Sensitivity: At 1.56 pg/mL, the kit detects IFNG in unstimulated canine plasma and resting-state tissue homogenates, where this cytokine is often near the lower limit of conventional assays — critical for establishing true baseline values before antigen challenge.

Sample Compatibility: Validation across serum, plasma, and tissue homogenates (50–100 µL per well) enables researchers to correlate systemic IFNG responses with localized tissue inflammation, supporting paired designs common in canine dermatology and organ-specific immunity studies.

Assay Efficiency: The 1–5 hour protocol and low sample volume accommodate limited-volume veterinary blood draws and time-sensitive ex vivo stimulation experiments using canine PBMCs.

This kit is well-suited for canine leishmaniasis immunomonitoring, vaccine immunogenicity assessment, and atopic dermatitis research.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.

Gene References into Functions

IL-17A gene transcription level was positively correlated with mRNA expression for iNOS and IFN-gamma PMID: 26590047
In dogs with leishmaniasis, there was high expression of interferon gamma in lymph nodes. PMID: 21511273
These findings suggest that polyunsaturated fatty acids are able to influence proliferation of peripheral blood mononuclear cells in healthy and atopic dogs but do not seem to influence cytokine transcription. PMID: 20187917

Subcellular Location

Secreted.

Protein Families

Type II (or gamma) interferon family

Tissue Specificity

Released primarily from activated T lymphocytes.

Database Links

KEGG: cfa:403801

STRING: 9615.ENSCAFP00000000588

UniGene: Cfa.3900

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Related pathways

HIF-1 signaling pathway Jak-STAT signaling pathway TGF-beta signaling pathway Necroptosis Antigen processing and presentation IL-17 signaling pathway Natural killer cell mediated cytotoxicity Osteoclast differentiation T cell receptor signaling pathway Th1 and Th2 cell differentiation Th17 cell differentiation

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