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Dog Interferon γ,IFN-γ ELISA Kit

Protocol MSDS
Code CSB-E11262c
Size 96T,5×96T,10×96T
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Product Details

Target Name
interferon, gamma
Alternative Names
IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
Abbreviation
IFNG
Uniprot No.
P42161
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 pg/mL-200 pg/mL
Sensitivity
0.78 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 95  
Range % 90-104  
1:2 Average % 96  
Range % 92-102  
1:4 Average % 95  
Range % 89-102  
1:8 Average % 97  
Range % 90-101  
Recovery
The recovery of dog IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 93-100  
EDTA plasma (n=4) 96 89-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 1.885 1.853 1.869 1.766  
100 1.226 1.308 1.267 1.164  
50 0.891 0.924 0.908 0.805  
25 0.636 0.686 0.661 0.558  
12.5 0.407 0.425 0.416 0.313  
6.25 0.310 0.318 0.314 0.211  
3.12 0.248 0.252 0.250 0.147  
0 0.102 0.104 0.103 　  
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Dog IFNG ELISA Kit was designed for the quantitative measurement of Dog IFNG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

Related Products

IFNG Proteins

IFNG ELISA kits

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Target Background

Function
(From Uniprot)
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
Gene References into Functions
  1. IL-17A gene transcription level was positively correlated with mRNA expression for iNOS and IFN-gamma PMID: 26590047
  2. In dogs with leishmaniasis, there was high expression of interferon gamma in lymph nodes. PMID: 21511273
  3. These findings suggest that polyunsaturated fatty acids are able to influence proliferation of peripheral blood mononuclear cells in healthy and atopic dogs but do not seem to influence cytokine transcription. PMID: 20187917
Subcellular Location
Secreted.
Protein Families
Type II (or gamma) interferon family
Tissue Specificity
Released primarily from activated T lymphocytes.
Database Links
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Related pathways
HIF-1 signaling pathway Jak-STAT signaling pathway TGF-beta signaling pathway Necroptosis Antigen processing and presentation IL-17 signaling pathway Natural killer cell mediated cytotoxicity Osteoclast differentiation T cell receptor signaling pathway Th1 and Th2 cell differentiation Th17 cell differentiation
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