Dog arginine vasopressin (AVP) ELISA kit

Instructions
Code CSB-EL002466DO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name arginine vasopressin (AVP)
Abbreviation AVP
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma
Detection Range 0.78 pg/mL-50 pg/mL
Sensitivity 0.2 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-94
1:2Average %96
Range %92-101
1:4Average %86
Range %81-92
1:8Average %90
Range %84-97
Recovery
The recovery of dog AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9187-96
EDTA plasma (n=4)107103-111
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
502.507 2.703 2.605 2.462
252.129 2.221 2.175 2.032
12.51.493 1.598 1.546 1.403
6.250.903 0.912 0.908 0.765
3.120.519 0.526 0.523 0.380
1.560.362 0.377 0.370 0.227
0.780.247 0.239 0.243 0.100
00.138 0.147 0.143
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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