Dog thrombomodulin,TM ELISA Kit

Code CSB-E06529c
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
thrombomodulin
Alternative Names
THBD ELISA Kit; Thrombomodulin ELISA Kit; TM ELISA Kit; CD antigen CD141 ELISA Kit
Abbreviation
THBD
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
23.44 pg/mL-1500 pg/mL
Sensitivity
5.86 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:10Average %97
Range %91-103
1:20Average %94
Range %91-98
1:40Average %90
Range %88-94
1:80Average %93
Range %89-97
Recovery
The recovery of dog TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8983-94
EDTA plasma (n=4)9387-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
15000.087 0.086 0.087
7500.148 0.142 0.145
3750.234 0.229 0.232
187.50.347 0.338 0.343
93.750.562 0.576 0.569
46.880.865 0.921 0.893
23.441.464 1.392 1.428
02.677 2.568 2.623
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Dog THBD ELISA Kit was designed for the quantitative measurement of Dog THBD protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

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Target Background

Function
(From Uniprot)
Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
Gene References into Functions
  1. plasma levels are higher in dogs infected with Leishmania infantum PMID: 15372989
  2. cDNA sequence analysis, amino acid sequence homology, and tissue expression. PMID: 15585960
Subcellular Location
Membrane; Single-pass type I membrane protein.
Tissue Specificity
Expressed in lung, liver, spleen, kidney, pancreas and lymph node. Low expression in heart, cerebrum, urinary bladder and uterus.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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