Fish Complement 3,C3 ELISA Kit

Code CSB-E09727s
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Fish Complement 3 (C3) ELISA Kit was designed for the quantitative measurement of Fish Complement 3 (C3) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 12.5 μg/mL-500 μg/mL and the sensitivity is 12.5 μg/mL.

Target Name Complement 3,C3
Abbreviation C3
Species Fish
Sample Types serum, plasma
Detection Range 12.5 μg/mL-500 μg/mL
Sensitivity 12.5 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 85-100  
1:2 Average % 96  
Range % 90-108  
1:4 Average % 95  
Range % 91-98  
1:8 Average % 95  
Range % 91-100  
Recovery
The recovery of fish C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-94  
EDTA plasma (n=4) 95 91-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
500 0.176 0.174 0.175    
250 0.381 0.393 0.387    
115 0.557 0.569 0.563    
37.5 0.894 0.901 0.898    
12.5 1.481 1.498 1.490    
0 2.417 2.542 2.480    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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