Fish Estradiol(E2) ELISA kit

Code CSB-E13017Fh
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Abbreviation
E2
Species
Fish
Sample Types
serum, plasma
Detection Range
40 pg/ml-1000 pg/ml
Sensitivity
40 pg/ml
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Citations

Customer Reviews and Q&A

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 Q&A
Q:

Hi, I need to get Testosterone ELISA kits to test for the levels in flounder (fish) blood plasma.

A:
Thanks for your inquiry.
Regarding Testosterone ELISA kit,we have the following item for fish.
#CSB-E17554Fh,Fish Testosterone(T) ELISA Kit
This is a kit of small molecule and are of wide applicability on fishes.While we did not ever use them to validate on flounder samples.We suggest you do pre-testing first and take the actual measurement as the real result.
Q:

I want to know if your Estradiol and testosterone assays can be done in gonad tissues. If so, how can I homogenate those tissues?

A:

May I know which species you need? human or mouse?
Here is the sample preparation method for tissue samples.
Tissue Homogenates 100mg tissue was rinsed with 1X PBS, homogenized in 1 ml of 1X PBS and stored overnight at -20°C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate was removed and assayed immediately. Alternatively, aliquot and store samples at -20°C or -80°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
After processing according to the above method, please be sure to do gradient pre-experiment.

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