Rabbit Estradiol,E2 ELISA Kit

Code CSB-E06915Rb
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation E2
Target Name Estradiol,E2
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma
Detection Range 62.5 pg/mL-1000 pg/mL
Sensitivity 62.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 82-91  
1:2 Average % 87  
Range % 84-90  
1:4 Average % 87  
Range % 83-91  
1:8 Average % 96  
Range % 93-99  
Recovery
The recovery of rabbit E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 91-97  
EDTA plasma (n=4) 101 98-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1000 0.204 0.206 0.205    
500 0.346 0.368 0.357    
250 0.602 0.642 0.622    
125 1.115 1.164 1.140    
62.5 2.342 2.357 2.350    
References
Still Have Questions? Leave a Message or Start an on-line Chat

Related Products

E2 ELISA Kit

E2 ELISA Kit for Bos taurus (Bovine)

E2 ELISA Kit for Canis lupus familiaris (Dog) (Canis familiaris)

E2 ELISA Kit for Fish

E2 ELISA Kit for Homo sapiens (Human)

E2 ELISA Kit for Mus musculus (Mouse)

E2 ELISA Kit for Rattus norvegicus (Rat)

E2 ELISA Kit for Ovis aries (Sheep)

E2 ELISA Kit for Gallus gallus (Chicken)

E2 ELISA Kit for Capra hircus (Goat)

E2 ELISA Kit for Equus caballus (Horse)

E2 ELISA Kit for Duck

E2 ELISA Kit for Monkey

E2 ELISA Kit for Sus scrofa (Pig)

E2 ELISA Kit for Guinea Sus scrofa (Pig)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.