Bovine Estradiol,E2 ELISA Kit

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Bovine Estradiol,E2 ELISA Kit

Name: Bovine Estradiol,E2 ELISA Kit
Brand: CUSABIO
SKU: CSB-E08173b

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Citations (14) Protocol

Code	CSB-E08173b
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (14)
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Alternative Names

N/A

Abbreviation

Species

Bos taurus (Bovine)

Sample Types

serum, plasma.

Detection Range

50 pg/ml-1200 pg/ml.

Sensitivity

40 pg/ml.

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

2.033

2.134

2.083

1.495

1.467

1.481

120

1.043

1.028

1.035

250

0.739

0.729

0.734

500

0.466

0.481

0.473

1200

0.256

0.253

0.255

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The Bovine estradiol (E2) ELISA Kit is ideal for the quantitative analysis of bovine estradiol in serum and plasma. This highly sensitive kit has a range of 50 pg/ml-1200 pg/ml and a sensitivity of 40 pg/ml. It uses a competitive detection mechanism, where the estradiol in the sample competes with a labeled estradiol for a limited number of antibody binding sites. The amount of labeled estradiol bound to the antibody inversely correlates with the concentration of estradiol in the sample, allowing for accurate quantification. The assay requires 50-100 μL of sample and has a duration of 1-5 hours, with results read at 450 nm wavelength. The kit has been used in more than 12 papers.

Apricot Bee Pollen Alleviates Deoxynivalenol-Induced Cellular Toxicity in Bovine Granulosa Cells C Lv, X Zheng, H Wu, P Sun, Q Lu,Animals: an Open Access Journal from MDPI,2025
Exploring the impact of exogenous Kisspeptin-10 on ovarian follicular development and estrus behaviour in Lakhimi cows of Assam A DATTA,/,2024
Conception rate according to the size and location of corpus luteum and coexistent follicle before embryo transfer in Hanwoo SG Lim,/,2024
Ovarian activity, hormone profile, pro‐inflammatory cytokines and reproductive performance of buffalo cows fed diets with different estrogenicity NM Hashem,Journal of animal physiology and animal nutrition,2023
Targeted metabolomics analysis of bile acids and cell biology studies reveal the critical role of glycodeoxycholic acid in buffalo follicular atresia Y Wei,Journal of steroid biochemistry and molecular biology,2023
Rumen-protected glucose stimulates the secretion of reproductive hormones and the mTOR/AKT signaling pathway in the ovaries of early postpartum Y Wang,researchsquare,2022
Association among lipopolysaccharide, the transforming growth factor-beta superfamily, follicular growth, and transcription factors in spontaneous bovine ovarian cysts Colakoglu H E, et al,Domestic Animal Endocrinology,2019
Circulating levels of adipokines and hormones in periparturient primiparous Sahiwal cows VIJAY PANDEY, et al,Indian Journal of Animal Sciences,2019
Effect of treatment with angiopoietin-2 and vascular endothelial growth factor on the quality of xenografted bovine ovarian tissue in mice Kong HS.et al,PLoS One.,2017
Saliva ferning, an unorthodox estrus detection method in water buffaloes (Bubalus bubalis). Ravinder R.et al,Theriogenology.,2016
Assessment of serum levels of osteopontin, selenium and prolactin in patients with psoriasis compared with healthy controls, and their association with psoriasis severity Toossi P. et al,Clin Exp Dermatol,2015
Treatment of clinical endometritis in dairy cows by previously used CIDR M Eslami et al,Theriogenology,2015
Tadalafil modulates aromatase activity and androgen receptor expression in a human osteoblastic cell in vitro model Aversa A. et al,J Endocrinol Invest.,2015
Correlation of Blood Flow of the Preovulatory Follicle to its Diameter and Endocrine Profile in Dairy Buffalo Varughese E et al,Reprod Domest Anim,2013

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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■ Q&A

I do have one more inquiry regarding the estrogen ELISA test CSB-E08173b. The detection range of the estrogen ELISA kit is 40-2000pg/ml, but in the test, some of the samples have values beyond the detection range i.e 2928 pg/ml, so what to say about these values beyond the detection range?

The mentioned case happens. When the sample content is higher than the highest limit of the kit, it needs dilutions and then the result needs to be multiplied by the dilution factor. As to this biomarker, usually, it does not need dilutions.

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