This human Estradiol (E2) ELISA kit employs the competitive inhibition enzyme immunoassay technique to quantitatively determine the human E2 content in serum and plasma, with a sensitivity of 40 pg/ mL. The kit has the advantages of high sensitivity, strong specificity, good repeatability, Intra-/inter-batch difference less than 15%, and so on. Estradiol is a steroid hormone secreted by the ovaries. The conversion of testosterone to estradiol is accomplished by the aromatase system. As the main estrogen, estradiol is responsible for regulating female characteristics, accessory organ maturation, and the menstrual cycle and promoting the production of the mammary duct system. Estradiol in men is essential for modulating libido, erectile function, and spermatogenesis. Plasma estradiol is a dynamic process, ovulating at its highest, close to 300pg/ mL. During this period, estradiol stimulates the proliferation of granulosa cells, increases the size of uterine glands, and sends a positive feedback signal to the hypothalamus, increasing luteinizing hormone.
Standards or samples are added to the microtiter plate wells pre-coated with goat-anti-human E2 antibody with the anti-mouse E2 antibody and HRP-conjugated E2. E2 in the sample or standards competes with the HRP labeled E2 for limited binding sites on the anti-mouse E2 antibody. These Ag-Ab complexes are captured by the antibody immobilized on the plate. The solution color turns blue after the addition of substrate A and B. The color reaction is immediately terminated after the addition of the stop solution. And the color changes from blue to yellow. The color intensity is opposite to the amount of E2 in the sample and measured by a Microplate reader at 450 nm.