Human Estradiol,E2 ELISA Kit

Instructions
Code CSB-E05108h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This human Estradiol (E2) ELISA kit employs the competitive inhibition enzyme immunoassay technique to quantitatively determine the human E2 content in serum and plasma, with a sensitivity of 40 pg/ mL. The kit has the advantages of high sensitivity, strong specificity, good repeatability, Intra-/inter-batch difference less than 15%, and so on. Estradiol is a steroid hormone secreted by the ovaries. The conversion of testosterone to estradiol is accomplished by the aromatase system. As the main estrogen, estradiol is responsible for regulating female characteristics, accessory organ maturation, and the menstrual cycle and promoting the production of the mammary duct system. Estradiol in men is essential for modulating libido, erectile function, and spermatogenesis. Plasma estradiol is a dynamic process, ovulating at its highest, close to 300pg/ mL. During this period, estradiol stimulates the proliferation of granulosa cells, increases the size of uterine glands, and sends a positive feedback signal to the hypothalamus, increasing luteinizing hormone.

Standards or samples are added to the microtiter plate wells pre-coated with goat-anti-human E2 antibody with the anti-mouse E2 antibody and HRP-conjugated E2. E2 in the sample or standards competes with the HRP labeled E2 for limited binding sites on the anti-mouse E2 antibody. These Ag-Ab complexes are captured by the antibody immobilized on the plate. The solution color turns blue after the addition of substrate A and B. The color reaction is immediately terminated after the addition of the stop solution. And the color changes from blue to yellow. The color intensity is opposite to the amount of E2 in the sample and measured by a Microplate reader at 450 nm.

Materials provided:

Alternative Names N/A
Abbreviation E2
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 40 pg/mL-1000 pg/mL
Sensitivity 40 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Materials provided
  • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit E2 antibody.
  • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
  • Anti-mouse E2 Antibody 1 x 6 ml -- Act as the detection antibody.
  • HRP-conjugate 1 x 6 ml -- Bind to the E2 antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
  • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
  • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
  • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
  • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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