Canine Estradiol,E2 ELISA Kit

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Product Details

Target Name

Estradiol,E2

Abbreviation

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Types

serum, plasma, tissue homogenates

Detection Range

40 pg/mL-1400 pg/mL

Sensitivity

25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-101
1:2	Average %	89
	Range %	84-98
1:4	Average %	96
	Range %	90-101
1:8	Average %	93
	Range %	87-98

Recovery

The recovery of canine E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	95	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Troubleshooting
and FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Check Code：

Code	CSB-E06846c
Size	96T,5×96T,10×96T
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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.